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以新牧1号苜蓿为研究材料,采用RT-PCR方法,设计特异引物,从新牧1号苜蓿中克隆获得MvDREB基因cDNA,测序结果该片段全长为651 bp,Genbank登录号为GU073286.生物信息学分析结果表明,该基因序列与紫花苜蓿核苷酸序列相似度达99%,编码216个氨基酸,MvDREB蛋白氨基酸序列分析比对显示该蛋白属于AP2/ERF家族的亲水性核蛋白,蛋白质分子量为24873.1,理论等电点pI为5.90,不稳定参数为45.07.
Using Xinmu No. 1 alfalfa as materials, the MvDREB cDNA was cloned from Xinmu No. 1 by RT - PCR and sequenced. The full - length cDNA of MvDREB was 651 bp and the GenBank accession number was GU073286. The results of the phylogenetic tree analysis showed that the sequence of the gene shared 99% similarity with the nucleotide sequence of alfalfa and encoded 216 amino acids. The amino acid sequence analysis of the MvDREB protein revealed that the protein belongs to the hydrophilic nucleoprotein of the AP2 / ERF family. The protein molecular weight Is 24873.1, the theoretical isoelectric point pI is 5.90, and the unstable parameter is 45.07.