论文部分内容阅读
目的检测急性特发性血小板减少性紫癜(ITP)患儿治疗前后外周血血小板相关抗体(PAIgG)和白细胞介素18(IL-18)的变化,分析IL-18和PAIgG在急性ITP患儿发病机制中的作用。方法用流式细胞仪检测外周血PAIgG表达含量,ELISA法检测血浆中IL-18的含量,血细胞分析仪计数血小板(PLT)数量。结果急性ITP组治疗前PLT数量明显低于治疗后及对照组,差别均有统计学意义(P<0.01),治疗后PLT数量与对照组比较,差别无统计学意义(P>0.05);治疗前PAIgG表达量明显高于治疗后及对照组,差别均有统计学意义(P<0.01),治疗后的PAIgG表达量高于对照组,差别有统计学意义(P<0.05);治疗前IL-18含量均低于治疗后及对照组,差别有统计学意义(P<0.01),治疗后与对照组比较,差别无统计学意义(P>0.05)。PAIgG在治疗前后与血小板含量均呈负相关(r值分别为-0.87,-0.81,P<0.05),而IL-18含量在治疗前后与血小板含量均呈正相关(r值分别为0.76,0.74,P<0.05),IL-18含量和PAIgG表达在治疗前后均呈负相关(r值分别为-0.81,-0.79,P<0.05)。结论急性ITP患儿外周血中IL-18含量在治疗前后和PAIgG表达量有较好的相关性,IL-18的检测能够反映出急性ITP患儿外周血中PAIgG表达量的变化,PAIgG及IL-18在急性ITP发病机制中发挥了重要作用。
Objective To investigate the changes of peripheral blood platelet-related antibody (PAIgG) and interleukin 18 (IL-18) in children with acute idiopathic thrombocytopenic purpura (ITP) before and after treatment, and to analyze the changes of IL-18 and PAIgG in children with acute ITP Mechanism of the role. Methods The expression of PAIgG in peripheral blood was detected by flow cytometry. The content of IL-18 in plasma was detected by ELISA, and the platelet count (PLT) by hematology analyzer. Results The number of PLT in acute ITP group was significantly lower than that in the control group after treatment (P <0.01). There was no significant difference in the number of PLT between the two groups after treatment (P> 0.05) The expression of PAIgG before treatment was significantly higher than that after treatment and the control group (P <0.01), and the PAIgG expression after treatment was higher than that of the control group (P <0.05) -18 content were lower than that of the control and the control group, the difference was statistically significant (P <0.01), after treatment compared with the control group, the difference was not statistically significant (P> 0.05). PAIgG was negatively correlated with platelet content before and after treatment (r values were -0.87, -0.81, P <0.05, respectively), while IL-18 content was positively correlated with platelet content before and after treatment (r values were 0.76, P <0.05). The levels of IL-18 and PAIgG were negatively correlated before and after treatment (r = -0.81, -0.79, P <0.05). Conclusion IL-18 levels in peripheral blood of children with acute ITP have a good correlation with the expression of PAIgG before and after treatment. The detection of IL-18 can reflect the change of PAIgG expression in peripheral blood of children with acute ITP, PAIgG and IL -18 plays an important role in the pathogenesis of acute ITP.