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利用液相色谱对邻硝基甲苯、间硝基甲苯、对硝基甲苯、硝基苯、1,3,5-三硝基苯、2,4-二硝基甲苯、2,6-二硝基甲苯、环三亚甲基三硝胺、环四次甲基四硝胺、2,4,6-三硝基甲苯10种硝基化合物进行了分离,研究了色谱柱温度、流动相成分和流速对分离效果的影响。升高色谱柱温度或加快流动相流速都会使化合物的分离度变差。流动相成分的变化对各化合物分析的影响较大,不仅会改变化合物的分离度,还会使吸收波长发生移动。色谱柱为Zorbax Eclipse XDB-C_(18)(4.6×250 mm,5μm),色谱柱温度≤30℃、流动相为甲醇:水=55:45(V/V)、流速在0.6~1.2 m L/min时10种硝基化合物可以达到较好的分离。
By using liquid chromatography, the properties of o-nitrotoluene, m-nitrotoluene, p-nitrotoluene, nitrobenzene, 1,3,5-trinitrobenzene, 2,4-dinitrotoluene, Toluene, trinuclear trinitrotoluene, cyclotetramethylnitrosamine and 2,4,6-trinitrotoluene were isolated. The effects of temperature, mobile phase composition and flow rate Effect on the separation effect. Increasing the column temperature or increasing the flow rate of the mobile phase deteriorates the resolution of the compound. Changes in mobile phase composition of each compound has a greater impact on the analysis, not only will change the resolution of compounds, but also to shift the absorption wavelength. The column was Zorbax Eclipse XDB-C 18 (4.6 × 250 mm, 5 μm). The temperature of the column was ≤30 ℃ and the mobile phase was methanol: water = 55:45 (V / V) / min when 10 kinds of nitro compounds can achieve better separation.