论文部分内容阅读
目的研究支气管哮喘(哮喘)炎症促进因子白细胞介素4(IL-4)和炎症抑制因子(IL-12)的变化趋势,相互关系及对气道炎症反应的影响。方法用卵蛋白(OVA)敏原制备wistar大鼠哮喘模型,在激发后1、8、24、48、96h及7、14d行支气管肺泡灌洗(BAL),测定支气管肺泡灌洗液(BALF)中炎症细胞总数与嗜酸细胞数(EOS);酶联免疫吸附法(ELISA)测定BALF中IL-4、IL-12的浓度。结果OVA激发后,气道出现明显的炎症反应。EOS计数1h上升,8h后迅速上升,24h达到高峰期,96h后开始回落,7～14d回落到略高于对照组;炎症细胞总数1h上升,8h后迅速上升,24～96h处于高峰期,7d回落到接近8h水平,14d接近基础水平。BALF中,IL-4在1h上升,8～96h处于高峰期,7～14d回落到基础水平;IL-12在1h上升,8处于低水平,24h上升,48～96h处于高峰期,7～14d回落到基础水平;EOS与IL-4有正相关性(r=0.74,P<0.001),与IL-12无相关性(r=-0.13,P>0.05),与IL-4/IL-12有密切相关性(r=0.85,P<0.001)。结论哮喘气道中存在IL-4/IL-12比值失衡,IL-4/IL-12比值对哮喘气道炎症反应起重要的调节作用。
Objective To study the changes of interleukin-4 (IL-4) and inflammation inhibitor (IL-12) in bronchial asthma (asthma) and their relationship with airway inflammatory response. Methods The asthma model of wistar rats was prepared with ovalbumin (OVA) sensitizer. Bronchoalveolar lavage (BAL) was performed at 1, 8, 24, 48, 96 and 7,14 d after challenge. The total number of inflammatory cells and eosinophils (EOS) were measured. The concentrations of IL-4 and IL-12 in BALF were determined by enzyme linked immunosorbent assay (ELISA). Results After OVA challenge, the airway showed obvious inflammatory reaction. The number of EOS increased at 1h, rapidly increased at 8h, peaked at 24h, then decreased at 96h, and then dropped back slightly after 7 ~ 14d. The total number of inflammatory cells increased at 1h, rapidly increased at 8h, peaked at 24 ~ 96h, Back to nearly 8h level, 14d close to the basic level. In BALF, IL-4 increased at 1h, peaked at 8 ~ 96h and returned to basal level at 7 ~ 14d. IL-12 increased at 1h, decreased at 8, increased at 24h, peaked at 48 ~ 96h, (R = -0.13, P> 0.05), but no correlation with IL-4 (r = -0.13, P> 0.05) There was a close correlation (r = 0.85, P <0.001). Conclusion There is imbalance of IL-4 / IL-12 in airway of asthma, and IL-4 / IL-12 ratio plays an important role in airway inflammation of asthma.