论文部分内容阅读
目的研究胃癌(GC)形成中环氧合酶-2(COX-2)的表达与其细胞凋亡、增殖的关系,探讨COX-2在GC形成中的可能作用机制。方法采用免疫组化(SABC)法和原位末端标记法(TUNEL)检测15例正常胃黏膜(normalgastricmucosa,NGM)、30例胃粘膜肠上皮化生(IM)、30例异型增生(Dys)和40例GC组织中COX-2、增殖细胞核抗原(PCNA)阳性表达和细胞凋亡情况。结果在NGM→IM→Dys→GC的形成过程中,COX-2的表达呈递增趋势,其阳性表达中GC组(67.5%)和NGM组(13.3%)及GC组和IM组(33.3%)差别均有显著性(P<0.05)。细胞凋亡指数(AI)在GC时最低(2.8±0.6),GC组AI显著低于其他各组(P<0.01)。细胞增殖指数(PI)则呈递增趋势,GC组与各组比较差异有显著性(P<0.01)。在不同程度癌前病变中COX-2的表达差异无显著性(P>0.05)。COX-2在低分化GC中表达显著高于高分化型。COX-2、PI与GC淋巴结转移、血管浸润均密切相关(P<0.05)。从NGM→IM,COX-2与AI呈正相关(r=0.55,P<0.05);从Dys→GC,二者与AI负相关(r=-0.56,P<0.05)。在GC形成整个过程中,COX-2与PI呈正相关(r=0.61,P<0.05)。结论在GC形成过程中,COX-2的表达呈递增趋势;COX-2的表达上调可能是GC形成的早期事件,且其表达水平和PI对评价GC的恶性程度、有无转移和预后有一定参考价值;COX-2在GC形成的不同?
Objective To investigate the relationship between cyclooxygenase-2 (COX-2) expression and apoptosis and proliferation in gastric cancer (GC) and to explore the possible mechanism of COX-2 in GC formation. Methods 15 cases of normal gastric mucosa (NGM), 30 cases of gastric mucosal intestinal metaplasia (IM), 30 cases of dysplasia (Dys) and 30 cases of gastric mucosa were examined by immunohistochemistry (SABC) and TUNEL COX-2, PCNA expression and apoptosis in 40 cases of GC. Results The expression of COX-2 showed an increasing trend during the formation of NGM → IM → Dys → GC. The positive expression of COX-2 in GC group (67.5%) and NGM group (13.3%) and GC group and IM group (33.3% The difference was significant (P <0.05). The apoptosis index (AI) was the lowest at GC (2.8 ± 0.6), AI in GC group was significantly lower than that in other groups (P <0.01). Cell proliferation index (PI) showed an increasing trend, GC group and each group difference was significant (P <0.01). There was no significant difference in the expression of COX-2 in different degree of precancerous lesions (P> 0.05). COX-2 expression in poorly differentiated GC was significantly higher than the well-differentiated. COX-2, PI and GC lymph node metastasis, vascular invasion were closely related (P <0.05). From NGM → IM, COX-2 was positively correlated with AI (r = 0.55, P <0.05); from Dys → GC, both were negatively correlated with AI (r = -0.56, P <0.05). In the whole process of GC formation, COX-2 was positively correlated with PI (r = 0.61, P <0.05). CONCLUSION: The expression of COX-2 is an increasing trend in the process of GC formation. The up-regulation of COX-2 may be an early event of GC formation. The expression level of COX-2 may be associated with the malignancy, metastasis and prognosis of GC Reference value; COX-2 in GC formation of different?