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生物湿地床作为富营养化水体生态修复技术的重要组成部分,具有良好的发展潜能,其应用的关键是优选适宜的植物种类,并维持较高的污水净化能力。目前,国内外对湿地床根系微生物的群落结构与优势种群组成及变化研究甚少。本文利用变性梯度凝胶电泳技术(DGGE)对再力花湿地床不同根系微生物群落结构进行了解析。结果表明,腐根和黄根间具有较高相似度种群,而黄根、腐根与幼根相似度较低。微生物种类在腐根根系最为丰富,幼根最少。Clavibacter、Clostridium、Mesoflavibacter、Brevundimonas、Chromobacterium在生物湿地床去除污染物的过程中起到重要作用,为主要功能菌群。研究结果对应用生物湿地床净化水体中氮、磷具有一定的参考价值和理论意义。
As an important part of ecological restoration technology of eutrophic water bodies, biological wetland beds have good potential for development. The key to their application is the selection of suitable plant species and the maintenance of high wastewater purification capacity. At present, there is little research on the community structure and dominant population composition and variation of the root microbes in wetland beds at home and abroad. In this paper, denaturing gradient gel electrophoresis (DGGE) was used to analyze the microbial community structure of different roots in the re-wetland wetland. The results showed that there was a high similarity population between the roots and the roots, whereas the roots and rot roots had lower similarity with the roots. Microbial species in the roots of the most abundant roots, young roots at least. Clavibacter, Clostridium, Mesoflavibacter, Brevundimonas and Chromobacterium play an important role in the biological wetland bed removal of pollutants and are the main functional flora. The results of this study have certain reference value and theoretical significance for the application of biological wetland to purify nitrogen and phosphorus in water.