支原体污染是人和动物细胞系体外培养中常见的问题,污染细胞系与未污染细胞系的形态学,酶细胞化学和免疫细胞化学分析等表型均无差异。支原体在细胞融合中却能严重地减少杂交细胞的产生,因而急需建立一个可靠的去除支原体的方法以排除它们对融合率的破坏 Mycoplasma contamination is a common problem in in vitro culture of human and animal cell lines. There is no difference in the phenotypes between the contaminating and unpolluted cell lines, the enzymatic cytochemistry and the immunocytochemical analysis. Mycoplasma in the cell fusion but can seriously reduce the generation of hybrid cells, and therefore urgently need to establish a reliable removal of mycoplasma method to rule out their destruction of the fusion rate