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目的:观察D-serine合成相关的丝氨酸消旋酶(SR)在大鼠和癫痫病人海马组织的分布与细胞定位特点。方法:成年和幼龄SD大鼠灌流固定、冰冻切片癫痫患者海马硬化石蜡切片,进行SR、SR/NeuN、SR/GFAP免疫荧光染色、激光共聚焦显微镜观察和计数分析。结果:在成年和幼龄大鼠海马的CA1-3和齿状回内均分布有大量SR免疫阳性细胞分布,SR免疫阳性反应主要定位于神经元和星形胶质细胞;与成年相比,幼龄大鼠海马内SR免疫阳性的神经元数量相似,但SR/GFAP阳性星形胶质细胞的数量较多;SR阳性细胞在癫痫患者海马内也有大量分布,其数量明显多于脑出血患者。结论:SR定位于大鼠和癫痫患者海马神经元和星形胶质细胞,其数量分布与发育年龄和癫痫疾病状态呈现一定的相关性,提示D-serine的产生可能参与海马的发育和癫痫疾病过程。
Objective: To observe the distribution and cellular localization of serine gyrase (SR) related to D-serine synthesis in hippocampus of rats and epileptic patients. Methods: The hippocampal sclerosing paraffin sections of SD and SD rats were fixed by perfusion and frozen sections, and SR, SR / NeuN, SR / GFAP immunofluorescence staining and laser scanning confocal microscopy were performed. RESULTS: A large number of SR immunoreactive cells were distributed in hippocampus CA1-3 and dentate gyrus of adult and young rats. SR immunoreactive positive cells mainly located in neurons and astrocytes. Compared with adult rats, The numbers of SR immunopositive neurons in the hippocampus of young rats were similar, but the number of SR / GFAP positive astrocytes was more. The number of SR positive cells in the hippocampus of epilepsy patients was also significantly higher than that in patients with cerebral hemorrhage . CONCLUSIONS: SR is localized in hippocampal neurons and astrocytes in rats and epilepsy. The correlation between the number distribution and the age of onset and the status of epilepsy suggests that D-serine may be involved in the development of hippocampus and epilepsy process.