如果药物是通过代谢形式呈现其细胞毒作用,那么以组织培养细胞的方法来筛选药物的细胞毒作用就有其局限性,因此过去曾认为,只有通过体内试验才能解决。鉴于已知环磷酰胺在体外无细胞毒作用,仅是通过体内肝微粒体酶转化为有活性的烷化基才呈现抗肿瘤作用。本文乃研究了一种新的组织培养方法,使癌细胞与环磷酰胺一起温育(37℃),此时由于肝微粒体酶促使环磷酰胺 If the drug shows its cytotoxic effects through metabolic means, then the cytotoxicity of drugs to screen cells for tissue culture has its limitations, and so it has been thought in the past that this can only be solved by in vivo testing. In view of the fact that cyclophosphamide is known to be cytotoxic in vitro, it exhibits antitumor effects only through the conversion of active in vivo hepatic microsomal enzymes into alkylated groups. This article is a study of a new tissue culture method, so that cancer cells incubated with cyclophosphamide (37 ℃), this time due to liver microsomal enzymes so cyclophosphamide