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以褶皱臂尾轮虫 (Brachionusplicatilis )为实验动物并设计不同脂肪源饵料 ,分别为 :面包酵母Saccharomycescerevisice(对照组 ) ,微绿球藻Nannochlorpsisoculata (A) ,5 %大豆磷脂 + 5 %鱼油 + 90 %面包酵母 (B) ,10 %鱼油 + 90 %面包酵母 (C)。结果表明 :(1)接受不同脂肪源的轮虫的脂肪酸组成显著不同 ,尤其是高度不饱和脂肪酸 (HUFA ,2 0C)含量存在显著差异 ,说明饵料中HUFA含量对轮虫体内相应脂肪酸的含量有显著影响。投喂饵料C的轮虫具有高含量的n - 3HUFA(2 6 .7% ) ,而对照组轮虫的n - 3HUFA只有 7.5 6 % ,所以投喂饵料C使轮虫的脂类HUFA水平得到了强化 ,提高了轮虫的营养价值。 (2 )轮虫脂类的HUFA水平不仅由饵料中脂类的相应脂肪酸组成决定 ,而且轮虫脂类HUFA的提高和强化效果与饵料中HUFA的化学形态密切相关。甘油三酯型饵料HUFA的强化轮虫效果高于磷脂型的HUFA。 (3)将富含HUFA的鱼油直接添加在干性饵料如面包酵母中 ,而不是通过对鱼油乳化后直接强化轮虫 ,同样能取得良好的强化效果 ,且操作简便 ,不易污染水质 ,适用于轮虫的规模生产
Brachionus plicatilis was used as experimental animal and different fat sources were designed, which were: Saccharomyces cerevisiae (control group), Nannochlorpsisoculata (A), 5% soy lecithin + 5% fish oil + 90% Baker’s yeast (B), 10% fish oil + 90% baker’s yeast (C). The results showed that: (1) The composition of fatty acids of rotifers with different fat sources were significantly different, especially the contents of HUFA and 20C were significantly different, indicating that there was Significantly affected. The rotifer fed diets C had high content of n - 3HUFA (26.7%), while the control group had only 7.5% of the n - 3HUFA, so fed diets C resulted in the lipid HUFA level of rotifer Strengthen, improve the nutritional value of rotifer. (2) The HUFA level of rotifer lipids is not only determined by the corresponding fatty acid composition of the lipid in the diet, but also the effect of HUFA on the increase and enhancement of HUFA is closely related to the chemical form of HUFA in the diet. The enhanced rotifer effect of the triglyceride HUFA was higher than that of the phospholipid HUFA. (3) The addition of HUFA-rich fish oil directly to dry bait, such as baker’s yeast, rather than the direct strengthening of rotifers by emulsifying fish oil, also provides good fortification and is easy to handle and does not contaminate water, Rotifer scale production