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目的探讨中药健胎液对子宫内膜着床微环境改善的可能分子机制。方法 90只受孕小鼠随机分为正常组、模型组、中药组各30只,模型组和中药组采用吲哚美辛皮下注射建立胚胎着床障碍小鼠模型。中药组从妊娠第1天开始用健胎液(144%无菌煎剂)灌胃,每天每只0.5ml;模型组和正常组每天用等量生理盐水灌胃。在妊娠第5天检测各组小鼠(每组10只)子宫内膜白介素-1β(IL-1β)、白介素1受体Ⅰ型(IL-1RⅠ)、白介素-6(IL-6)、白血病抑制因子(LIF)、集落刺激因子-1(CSF-1)mRNA水平,妊娠第8天检查各组小鼠(每组20只)子宫内胚胎发育情况,记录胚胎着床率和着床位点数。结果中药组和模型组的胚胎着床率、着床位点数同正常组比较显著降低(P<0.01),中药组胚胎着床率、着床位点数同模型组比较明显升高(P<0.05)。与正常组比较,模型组IL-1β、IL-1RⅠ、IL-6、LIF、CSF-1 mRNA含量均显著升高(P<0.01);与模型组比较,中药组IL-1β、IL-1RⅠ、IL-6、LIF、CSF-1 mRNA含量均显著降低(P<0.05或P<0.01)。结论中药健胎液通过降低胚胎着床障碍小鼠子宫内膜IL-1β、IL-1RⅠ、IL-6、LIF、CSF-1细胞因子的基因表达,改善子宫内膜微环境,促进着床。
Objective To investigate the possible molecular mechanism of Jingshang decoction on improving microenvironment of endometrial implantation. Methods Totally 90 pregnant mice were randomly divided into normal group, model group and traditional Chinese medicine group, and 30 mice in model group and Chinese herb group were injected subcutaneously with indomethacin. Chinese medicine group from the first day of pregnancy with Kin fetal fluid (144% sterile decoction) gavage, each 0.5ml per day; model group and normal group daily with the same amount of saline gavage. On day 5 of pregnancy, the mice in each group (10 in each group) were tested for the levels of endometrial interleukin-1β (IL-1β), interleukin-1 receptor type 1 (IL-1RⅠ), interleukin-6 (LIF) and CSF-1 mRNA levels. The development of intrauterine embryos in each group (20 in each group) was examined on the 8th day of gestation. The embryo implantation rate and the number of implantation sites were recorded. Results The embryo implantation rate and implantation site of TCM group and model group were significantly lower than those of normal group (P <0.01). The embryo implantation rate and implantation site of TCM group were significantly higher than those of model group (P <0.05). The levels of IL-1β, IL-1RⅠ, IL-6, LIF and CSF-1 mRNA in the model group were significantly increased compared with the normal group (P <0.01) , IL-6, LIF, CSF-1 mRNA were significantly decreased (P <0.05 or P <0.01). Conclusion JSTF can improve the endometrial microenvironment and promote implantation by reducing the gene expression of IL-1β, IL-1RⅠ, IL-6, LIF and CSF-1 cytokines in endometrium of embryo implantation dysfunction mice.