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目的:测定护肝颗粒中大黄素、大黄素甲醚的含量.方法:高效液相色谱法,KROMASIL C18(4－6MM *250MM ,5－0μM)色谱柱,流动相:甲醇－0－1％ 醋酸溶液(90:10),检测波长:290NM ,流速:1－0ML?MIN－1 ,柱温:40℃.结果:含量测定大黄素、大黄素甲醚的线性范围分别在0－07175~0－574μG、0－0458~0－366μG,大黄素平均回收率97－92 ％ , RSD 为1－06 ％ .大黄素甲醚平均回收率98－27％ ,RSD 为0－85％ .结论:建立的含量测定方法简便、快捷、灵敏、准确“,”AIM To determine emodin and physcion in Hugan granules METHOD High performance liquid chromatography was nsed,emodin and physcion were separated by Kromasil C 18 column (4 6mm*250mm,5μm) and detected at 290nm,Mobile phase was methanol-0 1% acetic acid solution(90:10),flow rate 1 0ml·min -1 ,column temperature 40℃.RESULTS The linear ranges of determination of emodin and physcion were 0 07175~0 574μg(r=0 9996) and 0 0458~0 366μg(r=0 9999),respectively.The meanrecovery of 97 92% (RSD=1 06%),for emodin and 98 27%(RSD=0 85%) for physcion was obtained.CONCLUSION This method was simple,high accuracy,and precision,and was sensitive and specific.