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目的对我国８个城市的４３株乙型肝炎表面抗原（ＨＢｓＡｇ）阳性和５２株ＨＢｓＡｇ阴性血清进行乙肝病毒（ＨＢＶ）ＤＮＡ扩增及其血清型和基因型分型，并对该两类血清的乙肝病毒型别构成及其地理分布进行比较。方法对长春、大同、杭州、深圳、青岛、西安、昆明和拉萨等８个城市共计４３例ＨＢ－ｓＡｇ阳性和５２例ＨＢｓＡｇ阴性的乙肝病人血清进行ＨＢＶＤＮＡＰＣＲ扩增（Ｓ基因第３３～５３３位核苷酸）并用ＰＣＲ扩增产物直接测序法分析ＨＢＶ血清型和基因型。结果ＨＢｓＡｇ阳性和阴性乙肝的ＨＢＶ血清型均以ａｄｒｑ＋为最多，分别为５８．１％和４０％；其次是ａｄｗ２，分别为２５．６％和３２．７％。ＨＢｓＡｇ阴性乙肝组ａｙｗ３（１９．２％）明显多于ＨＢｓＡｇ阳性乙肝组（２．３％，Ｐ＜０．０５）；而ａｙｗ１前者（０％）显著少于后者（１１．６％，Ｐ＜０．０１）。ＨＢｓＡｇ阳性和阴性乙肝的ＨＢＶ基因型均以Ｃ型为主，分别为６２．８％和６１．５％；其次为Ｄ型，分别为１４％和２６．９％；Ｂ型，分别为２０．９％和９．６％；Ａ型，各１株。各城市间两类乙肝的ＨＢＶ血清型和基因型存在一定差异。总体上看，两类乙肝的ＨＢＶ主要型别构成基本一致。结论ＨＢ－ｓＡｇ阴性乙肝?
OBJECTIVE To amplify hepatitis B virus (HBV) DNA from 43 hepatitis B surface antigen (HBsAg) positive and 52 HBsAg negative serum in 8 cities of China, and to identify the serotypes and genotypes of hepatitis B virus The composition of hepatitis B virus and its geographical distribution were compared. Methods A total of 43 HBsAg-positive and 52 HBsAg-negative hepatitis B patients from 8 cities including Changchun, Datong, Hangzhou, Shenzhen, Qingdao, Xi’an, Kunming and Lhasa were enrolled in this study. HBVDNAPCR amplification was performed in sera from 33 to 533 nuclei ) And HBV serotypes and genotypes were analyzed by direct sequencing of PCR amplification products. Results The HBV serotypes of HBsAg-positive and -negative hepatitis B were adrq +, which were 58.1% and 40% respectively, followed by adw2, which was 25.6% and 32.7% respectively. The ayw3 (19.2%) in HBsAg-negative hepatitis B group was significantly more than that in HBsAg-positive hepatitis B group (2.3%, P <0.05), while the former ayw1 (0%) was significantly less than the latter <0.01). HBV genotypes of HBsAg-positive and -negative hepatitis B were predominantly C-type with 62.8% and 61.5% respectively, followed by D-type with 14% and 26.9%, respectively, and B-type with 20 and 20 respectively. 9% and 9.6%; A type, each one. HBV serotypes and genotypes of two types of hepatitis B in different cities are different. Overall, the two main types of hepatitis B HBV constitute basically the same type. Conclusion HB-sAg negative hepatitis B?