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目的：研究蒿甲醚（Ａｒｔ）对血吸虫己糖激酶（ＨＫ）、磷酸葡萄糖异构酶（ＧＰＩ）和磷酸果糖激酶（ＰＦＫ）的影响。方法：感染血吸虫小鼠１次ｉｇＡｒｔ１００或３００ｍｇｋｇ－１，并分别于２４ｈ和４８ｈ剖杀取虫。按生成ＮＡＤＰＨ和耗用ＮＡＤＨ的方法测定上述３种酶活力。结果：感染鼠ｉｇ．Ａｒｔ３００ｍｇｋｇ－１后２４ｈ，♀、虫体ＨＫ活力抑制率分别为３３．３％和１３．７％，ＧＰＩ活力则分别抑制１４．７％和１７．５％，４８ｈ后，♀、虫体的ＧＰＩ活力抑制率分别为４６．２％和３２．９％。但Ａｒｔ剂量为１００或３００ｍｇｋｇ－１时，给药后２４ｈ和４８ｈ的♀虫ＰＦＫ抑制率分别为６４．９％和７１％，均明显高于虫的１６．３％和５４．２％。结论：ＰＦＫ可能是Ａｒｔ作用于血吸虫糖酶解途径的靶酶之一。
Objective: To study the effect of Artemether on Schistosoma hexokinase (HK), phosphoglucose isomerase (GPI) and phosphofructokinase (PFK). Methods: Infection of schistosomiasis mice once igArt100 or 300mgkg-1, respectively, and at 24h and 48h profile to take the worm. According to the method of generating NADPH and consuming NADH, the above three enzyme activities were measured. Results: Infection with ig. After treatment with Art300mgkg-1 for 24 h, the inhibitory rates of HK vitality were 33.3% and 13.7%, respectively. The GPI activity was inhibited by 14.7% and 17.5%, respectively. After 48 hours, GPI activity inhibition rates were 46.2% and 32.9% respectively. However, the PFK inhibition rates of Trichuris were 24.9% and 71% at Arterial doses of 100 or 300 mg kg-1 at 24 h and 48 h, respectively, which were significantly higher than that of pinworm at 16.3% and 54.2%, respectively. Conclusion: PFK may be one of the target enzymes Art plays in the enzymatic hydrolysis of Schistosoma japonicum.