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目的：研究内毒素休克时ＰＡＦ对ＴＮＦ、ＮＯ２／ＮＯ３的影响，探讨其可能的机理。材料与方法：将家兔随机分为５组，Ⅰ组：假手术对照组ｎ＝８；Ⅱ组：低剂量内毒素休克组ｎ＝１５，Ｅ．ｃｏｌｉ内毒素静脉一次性注射２００μｇ／ｋｇ；Ⅲ组：高剂量内毒素休克组ｎ＝１５，内毒素静脉一次性注射３００μｇ／ｋｇ；Ⅳ（或Ⅴ）组：低（或高）剂量内毒素并苦银杏内酯Ｂ预处理组ｎ＝１５，苦银杏内酯Ｂ５ｍｇ／ｋｇｉｐ注射，１０分钟后再分别静脉注射内毒素２００或３００μｇ／ｋｇ。于给药前、给药后即刻、３０、６０、１８０、３００、４８０分钟取血，测定血ＰＡＦ、ＴＮＦ、ＮＯ２／ＮＯ３。结果：内毒素休克时血浆ＰＡＦ、ＴＮＦα、ＮＯ２／ＮＯ３等均显著升高，且与内毒素的剂量密切相关。苦银杏内酯Ｂ预处理后对ＰＡＦ、ＴＮＦα的水平没有明显影响；对血浆ＮＯ２／ＮＯ３的升高有明显的抑制作用。苦银杏内酯Ｂ预处理后可使动物的存活率有较大的提高。结论：ＰＡＦ受体拮抗剂苦银杏内酯Ｂ可选择性地影响休克介质（如ＰＡＦ、ＴＮＦ、ＮＯ２／ＮＯ３等）的释放，提高动物的存活率。
Objective: To study the effect of PAF on TNF, NO2/NO3 and the possible mechanism of endotoxin shock. Materials and Methods: Rabbits were randomly divided into 5 groups: Group I: sham-operated control group n=8; Group II: low-dose endotoxic shock group n=15, E. Coli endotoxin intravenous injection of 200μg/kg; group III: high-dose endotoxic shock group n=15, endotoxin intravenous injection of 300μg/kg; IV (or V) group: low (or high) dose of endotoxin and Bitter ginkgolides B pretreatment group n=15, bitter bilobalide B5mg/kgip injection, and endotoxin 200 or 300μg/kg intravenously after 10 minutes. Immediately before and immediately after administration, blood was taken at 30, 60, 180, 300, and 480 minutes to determine blood PAF, TNF, and NO2-/NO3-. Results: Plasma levels of PAF, TNFα, and NO 2 -/NO 3 - were significantly increased in endotoxin shock, and were closely related to the dose of endotoxin. After treatment with bitter ginkgolide B, there was no significant effect on the levels of PAF and TNFα, and there was a significant inhibitory effect on the increase of plasma NO2/NO3. Pretreatment with bitter ginkgolide B can greatly improve animal survival. Conclusion: PAF receptor antagonist bitter bilobalide B can selectively affect the release of shock media (such as PAF, TNF, NO2 /NO3 etc.), and increase the survival rate of animals.