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①目的建立简易、实用的生物素－亲和素系统（ＡＢＣ）的免疫酶染色试验（ＡＢＣ－ＩＥＳＴ）和斑点酶联免疫吸附试验（ＡＢＣ－Ｄｏｔ－ＥＬＩＳＡ）的方法，以检测弓形虫病的微量抗体。②方法以全虫和可溶性虫体为抗原，在常规ＩＥＳＴ和Ｄｏｔ－ＥＬＩＳＡ的基础上，加用ＡＢＣ，观察其特异度和敏感度。③结果ＡＢＣ－ＩＥＳＴ和ＡＢＣ－Ｄｏｔ－ＥＬＩＳＡ的特异度和常规法相同，但敏感度有显著提高。④结论ＡＢＣ法可应用于微量抗体及早期感染的检测。
Objective To establish a simple and practical method for the detection of toxoplasmosis by ABC-IEST and ABC-Dot-ELISA (ABC-Dot-ELISA) Trace antibody. Methods All the worms and soluble parasites as the antigen, based on conventional IEST and Dot-ELISA plus ABC, to observe the specificity and sensitivity. ③ Results The specificity of ABC-IEST and ABC-Dot-ELISA was the same as that of the conventional method, but the sensitivity was significantly increased. ④ Conclusion ABC method can be applied to trace antibody and early detection of infection.