人γ-型干扰素(IFN-γ)基因工程研究是中国科学院上海生物化学研究所203课题组与复旦大学、第二军医大学等单位合作完成的.IFN-γ是一具有免疫活性的蛋白质,可能对某些肿瘤有治疗作用.本工作用人工合成的IFN-γ基因与表达载体重组构成表达质粒,转化大肠杆菌后,细菌产生的蛋白质中有60—80%为IFN-γ,这是国际上罕 Human γ-type interferon (IFN-γ) gene engineering research is Shanghai Institute of Biochemistry Chinese Academy of Sciences 203 task group and Fudan University, Second Military Medical University and other units completed .IFN-γ is an immunocompetent protein, May have therapeutic effect on some tumors.In this work, recombinant plasmids were constructed by recombination with synthetic IFN-γ gene and expression vector. After transformation of Escherichia coli, 60-80% of the proteins produced by bacteria were IFN-γ, On rare