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[Objective] This study is aimed to transform gus gene into Chinese cabbage (Brassica rapa pekinensis) by floral-dip method. [Method] The Chinese cabbage was transformed by floral-dip method for the first time. Then the cabbage seeds were harvested and screened by hygromycin, and the plants with resistance were confirmed by histochemical GUS assay and PCR detection. [Result] The target gene was successfully integrated into the Chinese cabbage genome, and the transformation rate was 0.1%. [Conclusion] This study optimized the genetic transformation system for Chinese cabbage, and laid the foundation for improving the means of genetic transformation of Chinese cabbage.
[Objective] This study is aimed at transforming gus gene into Chinese cabbage (Brassica rapa pekinensis) by floral-dip method. [Method] The Chinese cabbage was transformed by floral-dip method for the first time. Then the cabbage seeds were harvested and screened by hygromycin, and the plants with resistance were confirmed by histochemical GUS assay and PCR detection. [Result] The target gene was successfully integrated into the Chinese cabbage genome, and the transformation rate was 0.1%. [Conclusion] This study optimized the genetic transformation system for Chinese cabbage, and laid the foundation for improving the means of genetic transformation of Chinese cabbage.