The influence of polypropylene fiber on the flexural fatigue performance of high- strength concrete (HSC), which could be used as cover of reinforcement of segment, was investigate by three-point load bending tests. Also, the flexural fatigue equations of high-strength concrete with and without polypropylene fiber were established through test analysis. The experimental results indicate that the addition of polypropylene fiber can improve the static bending strength of segment concrete, and the important is that it can markedly increase the flexural fatigue performance of the HSC subjected to cyclic bending load. Especially when with 1.37 kg/m3 addition of the fiber was corporate with silica fume and slag powder, the fatigue life of the HSC can be increased by 43.4% compared to that of the segment concrete without fiber,silica fume and slag.