论文部分内容阅读
本实验研究了P物质(SP)与生长抑素(SOM)在体外对人脾脏中NK活性和巨噬细胞(Mφ)杀瘤活性的调节作用。结果显示,0.0001μmol/L的SP在效靶比例为100:1、50:1和25:1时能显著增强NK活性。仅在效靶比例为100:1时,0.01μmol/L的SP能显著增强NK活性。0.0001或O.01μmol/L的SP可使Mφ杀瘤活性升高,但作用不明显。1φmol/l的SP对NK活性和Mφ杀瘤活性均未显示出增强作用。与之相反,0.01或1μmol/L SOM在各效靶比例时都可显著抑制NK活性,并显著抑制Mφ杀瘤活性,而0.0001μmol/L SOM对两种活性均无抑制作用。SP或SOM在0.01及1μmol/L时,对200U/mlγ干扰素增强NK活性Mφ杀瘤活性的效应有降低作用。这些结果表明,SP及SOM参与调节NK活性和Mφ杀瘤活性,提示神经内分泌递质在机体抗肿瘤免疫中是重要的调节物质。
This study investigated the regulation of NK activity and macrophage (Mφ) cytotoxic activity in human spleens by substance P (SP) and somatostatin (SOM) in vitro. The results showed that 0.0001μmol / L SP significantly enhanced NK activity at target ratios of 100: 1, 50: 1 and 25: 1. Only at a target ratio of 100: 1, 0.01 μmol / L SP significantly enhanced NK activity. 0.0001 or 0.01μmol / L of SP can Moc tumor activity increased, but the effect is not obvious. SP at 1 mol / l did not show an enhancement effect on both NK activity and Mφ antitumor activity. In contrast, 0.01 or 1 μmol / L SOM significantly inhibited NK activity and significantly inhibited Mφ cytotoxicity at each target ratio, whereas 0.0001 μmol / L SOM did not inhibit both activities. The effect of 200 U / ml interferon-gamma on enhancing NK activity Mφ cytotoxicity activity was reduced at SP and SOM levels of 0.01 and 1 μmol / L. These results indicate that SP and SOM are involved in the regulation of NK activity and Mφ cytotoxic activity, suggesting that neuroendocrine neurotransmitters are important regulators in the body’s anti-tumor immunity.