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应用３Ｈ－胸腺嘧啶核苷（３Ｈ－ＴｄＲ）掺入法和四甲基偶氮唑ＭＴＴ比色法，探讨了人重组白细胞介素１（ｉｎｔｅｒｌｅｕｋｉｎ－１，ＩＬ－１）和白细胞介素６（ｉｎｔｅｒｌｅｕｋｉｎ－６，ＩＬ－６）对成人肾小球系膜细胞增殖的影响。结果发现，在无血清培养条件下，γＩＬ－１（ｒｅｃｏｍｂｉｎａｎｔＩＬ－１）和γＩＬ－６（ｒｅｃｏｍｂｉｎａｎｔＩＬ－６）均不能刺激系膜细胞的繁殖；而在有少量胎牛血清存在的条件下，γＩＬ－６可以明显地促进系膜细胞增殖，但γＩＬ－１无此作用。我们的实验还表明，γＩＬ－１和γＩＬ－６均能促进系膜细胞合成ＰＡＦ。ＩＬ－１和ＩＬ－６的这些作用提示，它们在肾小球免疫病理损伤过程中可能发挥重要作用。ＧｒｏｕｐＢ表示培养液中含低浓度血清（５％ＦＣＳ）表２ＩＬ－１对系膜细胞增殖的影响（ＭＴＴ法）ＧｒｏｕｐＡ、ＧｒｏｕｐＢ意义同表１３Ｈ－ＴｄＲ掺入法及ＭＴＴ法的结果均显示，无论培养基中有无血清存在，γＩＬ－１在一个较大的浓度范围内（１０～１００Ｏｕ／ｗｅｌｌ），都不能促进系膜细胞增殖（与对照组相比Ｐ＞０．０５）。２．２ＩＬ－６对系膜细胞增殖的影响研究发现，在无血清培养条件下，γＩＬ－６并不表现促细胞生长的作用?
The effects of human recombinant interleukin-1 (IL-1) and interleukin-6 (IL-1) interleukin-6, IL-6) on the proliferation of adult glomerular mesangial cells. The results showed that, γIL-1 (recombinant IL-1) and recombinant IL-6 could not stimulate the proliferation of mesangial cells in serum-free culture conditions; while in the presence of a small amount of fetal bovine serum, 6 can obviously promote mesangial cell proliferation, but γIL-1 does not have this effect. Our experiments also showed that both γIL-1 and γIL-6 promote mesangial cells to synthesize PAF. These effects of IL-1 and IL-6 suggest that they may play an important role in glomerular immunopathological damage. GroupB indicates that culture medium contains low concentration of serum (5% FCS) Table 2 IL-1 on mesangial cell proliferation (MTT method) GroupA, GroupB Significance The same table 13H-TdR incorporation and MTT method results show that no matter Culture medium with or without serum, γIL-1 in a large concentration range (10 ~ 100Ou / well), can not promote mesangial cell proliferation (compared with the control group P> 0.05). 2.2 IL-6 mesangial cell proliferation study found that serum-free culture conditions, γIL-6 does not show the role of cell growth?