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目的研究代谢综合征(MS)患者血清磷脂脂肪酸谱与核因子-κB(NF-κB)表达的相关性及其意义。方法以60例MS患者和60名健康对照(NC)者为研究对象,采用ELISA法测定血清中NF-κB的表达,高效气相色谱法测定血清磷脂脂肪酸谱。采用t检验进行组间比较分析,用Pearson相关性分析来分析血清磷脂脂肪酸谱和NF-κB的相关性。结果 MS组NF-κB表达为(11.66±3.63)ng/ml,明显高于NC组(P<0.01)。MS组的饱和脂肪酸(SFA)含量为(41.59±3.51)%,较NC组显著升高(P<0.01),多不饱和脂肪酸(PUFA)含量为(47.17±2.51)%,较NC组显著降低(P<0.01),2组之间的单不饱和脂肪酸(MUFA)无统计学差异。Pearson相关性分析显示,MS组中NF-κB的表达与SFA呈显著正相关(r=0.42,P=0.00),与n-6 PUFA、n-3 PUFA呈显著负相关(r=-0.33,-0.19,P=0.00,0.03)。结论 MS患者的血清磷脂脂肪酸构成中SFA比例增加,PUFA比例减少,且与NF-κB表达增加有关,提示膳食结构中SFA增多、PUFA减少可能通过NF-κB通路的激活而引起或加重MS的炎症状态。
Objective To study the correlation between the serum phospholipid fatty acid profile and the expression of nuclear factor-κB (NF-κB) in patients with metabolic syndrome (MS) and its significance. Methods Sixty patients with MS and 60 healthy controls (NC) were enrolled in this study. The expression of NF-κB in serum was measured by ELISA, and the serum phospholipid fatty acid profiles were determined by high performance gas chromatography. T-test was used to compare between groups, Pearson correlation analysis was used to analyze the correlation between serum phospholipid fatty acid profiles and NF-κB. Results The expression of NF-κB in MS group was (11.66 ± 3.63) ng / ml, which was significantly higher than that in NC group (P <0.01). The content of SFA in MS group was (41.59 ± 3.51)%, which was significantly higher than NC group (P <0.01) and PUFA content was (47.17 ± 2.51)%, which was significantly lower than that in NC group (P <0.01). There was no significant difference between the two groups in the content of monounsaturated fatty acids (MUFA). Pearson correlation analysis showed that there was a significant positive correlation between the expression of NF-κB and SFA in MS group (r = 0.42, P = 0.00) and negative correlation with n-6 PUFA and n-3 PUFA -0.19, P = 0.00, 0.03). Conclusions MS patients’ serum phospholipid fatty acid composition of SFA increased proportion of PUFA decreased, and NF-κB expression increased, suggesting that the dietary structure increased SFA, PUFA decreased NF-κB pathway may be caused by activation or increase the inflammation of MS status.