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在氧钒离子单独作用和氧钒离子与过氧化氢同时作用于环核苷酸时,用化学测磷法测定产生的PO_4~(3-)以确定环核苷酸的两个磷酯键是否同时断裂;用HPLC结合ESR等谱学方法来判断是否有一个酯键断裂的不对称水解发生.发现氧钒离子单独存在时并不能使环核苷酸的磷酸酯键水解;而在氧钒离子和过氧化氢共存时可使环核苷酸的磷酸二酯键中一个酯键水解成核苷单磷酸,且cAMP和cGMP在水解速度及生成3＇-或5＇-AMP和GMP的比例也不相同.
In the presence of vanadate ion alone and simultaneously with vanadyl ion and hydrogen peroxide on the cyclic nucleotide, the resulting phosphate 4 ~ (3-) is determined by chemical phosphorus determination to determine whether two phosphatidyl esters of cyclic nucleotides are present And break down at the same time.Using HPLC and ESR isotherm to determine whether there is an asymmetric hydrolysis of the ester bond.It was found that the presence of vanadyl ion alone did not hydrolyze the phosphate bond of the cyclic nucleotide, The coexistence of hydrogen peroxide and hydrogen can make a cyclic nucleotide phosphodiester bond hydrolysis of an ester bond to nucleoside monophosphate, and cAMP and cGMP in the hydrolysis rate and the formation of 3’- or 5’-AMP and GMP ratio Are not the same.