背景:白花败酱草为败酱科植物,味辛、苦性寒,已证实白花败酱草提取物具有中枢抑制作用。目的:观察中药白花败酱草提取物对小鼠耐缺氧的作用,并了解其作用是否有剂量依赖性。设计:随机对照动物实验。单位:赣南医学院药理教研室和病理教研室。材料:实验于2005-03/04在赣南医学院科研中心实验室完成。取健康成年昆明种小鼠100只,用于3个缺氧实验。方法:①常压耐缺氧实验:取小鼠40只随机分为4组:生理盐水组腹腔注射生理盐水2μL/g,普萘洛尔组腹腔注射10g/L普萘洛尔溶液0.02mg/g,白花败酱草0.02,0.04mg/g组腹腔注射白花败酱草提取物0.02,0.04mg/g。25min后,将小鼠放入250mL的磨口广口瓶内,密封,观察小鼠的存活时间。②快速断头实验:取小鼠30只随机分为3组:生理盐水组腹腔注射生理盐水2μL/g,白花败酱草0.02,0.04mg/g组分别于腹腔注射白花败酱草提取物0.02,0.04mg/g,25min后,快速断头,记录小鼠断头后至最后一次喘息所需的时间。③结扎双侧颈总动脉实验:取小鼠30只随机分为3组:生理盐水组灌胃生理盐水2μL/g,白花败酱草组0.01,0.015mg/g灌胃,1次/d,共7d,7d后结扎双侧颈总动脉观察呼吸停止的时间。主要观察指标:①小鼠常压耐缺氧的存活时间。②小鼠脑缺血断头后至最后一次喘息的时间。③小鼠结扎双侧颈总动脉至呼吸停止的时间。结果:100只小鼠全部进入结果分析。①常压耐缺氧条件下鼠的存活时间:白花败酱草0.02,0.04mg/g组显著长于生理盐水组[(57.8±4.6),(76.2±4.9),(42.5±3.6)min,P<0.05,0.01],与普萘洛尔组比较无差异(P>0.05),剂量越大,存活时间越长。②断头小鼠的喘息时间:白花败酱草0.02,0.04mg/g组显著长于生理盐水组[(22.1±1.6),(25.3±2.2),(18.6±0.8)s,P<0.05,0.01],剂量越大,存活时间越长。③结扎双侧颈总动脉小鼠呼吸停止的时间:白花败酱草提取物0.01,0.015mg/g组显著长于生理盐水组[(123.4±25.1),(142.2±30.2),(86.0±12.8)s,P<0.05,0.01],剂量越大,存活时间越长。结论:白花败酱草提取物对脑缺氧、全身性缺氧时所致心肌缺氧及心肌耗氧增加引起的心肌缺氧有明显的改善作用,且剂量越大,效果越显著。可能是白花败酱草提取物能改善心肌及脑耗氧量的结果。 Background: Paeonia lactiflora is a plant of Paeniaceae, which is spicy and bitterly cold. It has been confirmed that Paeonia extract has a central inhibitory effect. OBJECTIVE: To observe the anti-anoxia effect of Paeoniae Radix extract on mice and to understand whether the effect is dose-dependent. Design: Randomized controlled animal experiments. Unit: Department of Pharmacology and Pathology, Wannan Medical College. MATERIALS: The experiment was performed at the Laboratory of Scientific Research Center of Wannan Medical College from March to April 2004. One hundred healthy healthy Kunming mice were used for 3 hypoxic experiments. METHODS: 1 Atmospheric hypoxia tolerance test: 40 mice were randomly divided into 4 groups: normal saline group was intraperitoneally injected with 2 μL/g saline, and propranolol group was intraperitoneally injected with 10 g/L propranolol solution at 0.02 mg/ml. g, 0.02, 0.04 mg/g of Paeonia serrata 0.02,0.04 mg/g group was injected intraperitoneally with Patrinia variegata extract. After 25 min, the mice were placed in a 250 mL ground mouth jar, sealed, and the mice were observed for survival. 2 Rapid decapitation experiments: 30 mice were randomly divided into 3 groups: normal saline group was intraperitoneally injected with 2 μL/g physiological saline, Paeonia variegata 0.02, and 0.04 mg/g group were intraperitoneally injected with Paeoniae Radix extract 0.02 respectively. , 0.04mg/g, after 25min, quickly decapitated and recorded the time required after the mice were decapitated until the last wheezing. 3 Ligation of bilateral common carotid artery experiments: 30 mice were randomly divided into 3 groups: normal saline group gavage normal saline 2μL/g, Baihua Patrinia group 0.01, 0.015mg/g gavage, 1 time/d, After a total of 7 days and 7 days, bilateral common carotid artery was ligated to observe the time of respiratory arrest. MAIN OUTCOME MEASURES: 1 The survival time of mice under normal pressure and hypoxia. 2 The time from the end of the cerebral ischemia to the last wheezing. 3 The time when the bilateral carotid arteries were ligated to stop breathing. Results: 100 mice were all involved in the result analysis. (1) Survival time of rat under hypobaric and hypoxic conditions: The 0.02, 0.04 mg/g of Paeonia lactiflora was significantly longer than that of saline group [(57.8±4.6), (76.2±4.9), (42.5±3.6) min, P> <0.05, 0.01], compared with propranolol group no difference (P> 0.05), the greater the dose, the longer the survival time. The time of wheezing in 2 mice with decapitation was significantly longer in the 0.02 and 0.04 mg/g groups than in the saline group [(22.1±1.6), (25.3±2.2), (18.6±0.8) s, P<0.05, 0.01 ] The greater the dose, the longer the survival time. 3The time of respiratory arrest in mice with bilateral common carotid artery ligation was 0.01 in the extract of Patrinia violaceum, and the 0.015 mg/g group was significantly longer than the saline group [(123.4±25.1), (142.2±30.2), (86.0±12.8). s, P<0.05, 0.01], the larger the dose, the longer the survival time. Conclusion: The extract of Patrinia violacea extract can significantly improve myocardial hypoxia induced by hypoxia, hypoxia and myocardial oxygen consumption caused by hypoxia, and the greater the dose, the more significant the effect. It may be the result that the extract of Patrinia villosa can improve myocardial and brain oxygen consumption.