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目的建立同时测定芍药苷、阿魏酸、芸香柚皮苷、柚皮苷、新橙皮苷、甘草酸含量的超高效液相色谱(UPLC)法,研究柴胡疏肝散水提物的肠吸收特性。方法采用大鼠在体单向肠灌流和外翻肠囊实验模型,应用UPLC法测定柴胡疏肝散水提物中指标性成分(芍药苷、阿魏酸、芸香柚皮苷、柚皮苷、新橙皮苷和甘草酸)在不同时间点、不同部位肠道吸收量,计算吸收动力学参数,考察其肠吸收特征。结果在体单向肠灌流模型结果表明,芍药苷、芸香柚皮苷、新橙皮苷、柚皮苷和甘草酸均为中等程度吸收的化学成分,而阿魏酸为完全吸收;外翻肠囊模型结果表明,芍药苷在空肠部位,阿魏酸、柚皮苷和新橙皮苷在十二指肠部位,芸香柚皮苷在十二指肠和空肠部位的吸收最佳,而甘草酸在各肠段的吸收无显著性差异。结论肠道对柴胡疏肝散中6种指标性成分均有吸收,阿魏酸较其他5种指标性成分更易透过肠壁进入血液循环;不同肠段对6种指标性成分的吸收具有选择性。
OBJECTIVE To establish a method for the simultaneous determination of paeoniflorin, ferulic acid, rutin, naringin, naringin, neohesperidin and glycyrrhizic acid by ultra performance liquid chromatography (UPLC) characteristic. Methods The experimental model of rat intestinal peritoneal perfusion and valgus gut was established. The index components (paeoniflorin, ferulic acid, rutin, naringin, Neohesperidin and glycyrrhizin) at different time points, different parts of the intestinal absorption, calculation of absorption kinetics parameters to investigate the intestinal absorption characteristics. Results The unilateral intestinal perfusion model showed that paeoniflorin, rutin, naringin, neohesperidin, naringin and glycyrrhizin were all moderately absorbed chemical components, while ferulic acid was completely absorbed; Cystic model results showed that in the jejunal part of paeoniflorin, ferulic acid, naringin and neohesperidin in the duodenum, rutin naringin best absorption in the duodenum and jejunum parts, and glycyrrhizic acid There was no significant difference in intestinal absorption. Conclusions The intestinal tract can absorb 6 kinds of index components of Chaihu Shugan Powder, while ferulic acid can enter blood circulation more easily than other 5 kinds of index components. The absorption of 6 kinds of index components in different intestine segments has Selective.