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[目的]研究短梗胡枝子(Lespedeza cyrtobotrya)的组织培养。[方法]以短梗胡枝子种子为材料,对其组织培养进行了系统研究。[结果]2.1%次氯酸钠灭菌8min,发芽率较高,且污染率为0;在初代培养中,基础培养基为MS,6-BA对分化系数影响达到显著水平,适宜的增殖培养基为MS+1.00mg/L6-BA+0.10mg/LNAA+0.01mg/L2,4-D,分化系数可达6.69;在继代培养中,适宜的培养基为MS+1.00mg/L6-BA+0.05mg/L2,4-D;在生根培养中,IBA浓度为0.50mg/L时,生根率和平均生根数都最佳。[结论]为进一步开展胡枝子基因工程遗传改良提供了理论依据。
[Objective] The research aimed to study the tissue culture of Lespedeza cyrtobotrya. [Method] With Lespedeza stalon seeds as material, the tissue culture was systematically studied. [Result] 2.1% sodium hypochlorite was sterilized for 8min, the germination rate was high and the contamination rate was 0. In the primary culture medium, the basic culture medium was MS, and the effect of 6-BA on the differentiation coefficient reached a significant level. The suitable proliferation medium was MS In the subculture, the suitable culture medium was MS + 1.00 mg / L 6-BA + 0.05 mg / L 6-BA + 0.10 mg / L NAA + 0.01 mg / / L2,4-D. In the rooting culture, the rooting rate and average rooting number were the best when IBA concentration was 0.50mg / L. [Conclusion] This provided a theoretical basis for further genetic improvement of Lespedeza bicolor L. genetic engineering.