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目的了解江门市区2004-2008年食品中沙门菌、金黄色葡萄球菌、单核细胞增生李斯特菌、副溶血性弧菌及肠出血性大肠肝菌(EHEC)O157∶H7的污染状况,确定上述致病菌可能污染的高危食品,为食源性疾病监测提供科学依据。方法依据国标方法,并按《广东省食源性致病菌监测计划》检测技术要求,对采集的食品样本分别进行沙门菌、金黄色葡萄球菌、单核细胞增生李斯特菌、副溶血性弧菌及EHECO157∶H7分离、生化及血清学鉴定。结果从9类626份食品中,5年共检出致病菌54株,总检出率为8.63%。其中金黄色葡萄球菌3年检出14株,检出率最高为4.83%;其次为沙门菌5年检出23株,检出率为3.67%;单核细胞增生李斯特菌5年检出16株,检出率为2.56%;副溶血性弧菌5年中仅检出1株,检出率为0.16%;未检出EHECO157∶H7。以非定型包装熟肉、生肉类污染较为严重。结论应加强非定型包装熟肉和生肉类食品食源性致病菌污染的监测。
Objective To understand the contamination status of Salmonella, Staphylococcus aureus, Listeria monocytogenes, Vibrio parahaemolyticus and enterohemorrhagic Escherichia coli (EHEC) O157: H7 in food from 2004 to 2008 in Jiangmen city. The above-mentioned high-risk foods that may be contaminated by pathogens provide a scientific basis for the monitoring of food-borne diseases. Methods According to the national standard method and according to the technical requirements of “foodborne pathogens surveillance program in Guangdong Province”, the collected food samples were respectively subjected to Salmonella, Staphylococcus aureus, Listeria monocytogenes, Isolation of bacteria and EHECO157: H7, biochemical and serological identification. Results Among 626 kinds of food in 9 categories, 54 strains of pathogens were detected in 5 years, the total detection rate was 8.63%. Staphylococcus aureus detected 14 strains in 3 years, the highest detection rate was 4.83%; followed by Salmonella 23 strains detected in 5 years, the detection rate was 3.67%; Listeria monocytogenes detected 5 years 5 The detection rate was 2.56%. Only one strain of V. parahaemolyticus was detected in 5 years. The detection rate was 0.16%. EHECO157:H7 was not detected. Non-stereotypes packaged cooked meat, raw meat pollution is more serious. Conclusion The monitoring of food-borne pathogens contamination in cooked and uncooked foods should be strengthened.