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为探讨日本血吸虫重组蛋白LHD-Sj23-GST与3种不同类型佐剂(弗氏佐剂、ISA 206佐剂、ISA70M佐剂)配伍后免疫小鼠诱导的细胞免疫应答及免疫保护效果,应用流式细胞术检测并比较该重组蛋白配伍3种佐剂3次免疫后小鼠脾细胞中的CD4+、CD8+T细胞及细胞因子IFN-γ、IL-4、IL-10水平;3次免疫后小鼠人工攻击感染日本血吸虫尾蚴,6周后经肝门静脉灌注冲虫,计数各组平均虫体数并计算减虫率。与PBS对照组相比,LHD-Sj23-GST/FA和LHD-Sj23-GST/70M免疫组的CD4+T细胞显著升高(P<0.05),LHD-Sj23-GST/206免疫组未呈现明显变化;3种不同佐剂配伍LHD-Sj23-GST免疫组CD8+T细胞水平均显著降低(P<0.05);与PBS对照组相比,免疫组的IFN-γ及IL-10水平升高,而IL-4水平降低。LHD-Sj23-GST/FA、LHD-Sj23-GST/206和LHD-Sj23-GST/70M免疫组与PBS对照组相比,分别获得65.52%、51.72%和51.72%的减虫率;而相较于各自的佐剂对照组,则分别获得58.76%,26.31%,54.28%的减虫率。结果表明,用重组蛋白LHD-Sj23-GST配伍3种不同类型佐剂免疫小鼠,均刺激产生了偏向Th1型的细胞免疫应答,且都对小鼠诱导了较高的免疫保护作用。
In order to investigate the cellular immune response and immunoprotective effect induced by immunized mice after compatibility of recombinant protein LHD-Sj23-GST of Schistosoma japonicum with three different types of adjuvants (Freund’s adjuvant, ISA 206 adjuvant and ISA70M adjuvant) The levels of CD4 +, CD8 + T cells and cytokines IFN-γ, IL-4 and IL-10 in splenocytes of mice after 3 immunizations with 3 kinds of adjuvants were detected and compared by cytometry. After 3 immunizations The mice were challenged by artificial challenge with Schistosoma japonicum cercariae. After 6 weeks, the mice were perfused with hepatic portal vein and the average number of worms in each group was counted and the worm reduction rate was calculated. Compared with the PBS control group, CD4 + T cells in LHD-Sj23-GST / FA and LHD-Sj23-GST / 70M immunized groups were significantly increased (P <0.05), LHD-Sj23-GST / 206 immunized group showed no significant (P <0.05). Compared with the PBS control group, the levels of IFN-γ and IL-10 in the immunized group were increased, and the levels of CD8 + T cells in the three different adjuvants with and without LHD- While IL-4 levels decreased. Compared with the PBS control group, the worm reduction rates of LHD-Sj23-GST / FA, LHD-Sj23-GST / 206 and LHD-Sj23-GST / 70M groups were 65.52%, 51.72% and 51.72% In the respective adjuvant control groups, worm reduction rates of 58.76%, 26.31% and 54.28% were obtained respectively. The results showed that immunization of mice with the recombinant protein LHD-Sj23-GST combined with three different types of adjuvants stimulated a Th1-type cellular immune response, and all of them induced a higher immunoprotective effect in mice.