论文部分内容阅读
A computer-assisted method is presented for optimization of mobile phase compositions and development distance in gradient two-step development HPTLC. The method is based on a system which can predict the final Rf values for gradient two-step development from values measured using five preliminary runs. The statistical scanning method is then used for optimization, using Rf difference as the selection criterion. The method was evaluated using a mixture of eight components. Excellent agreement was obtained between predicted and experimental results. Gradient two-step development HPTLC, mobile phase composition optimization, velopment distance optimizationde-