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目的 :研究从黄芩中提取获得的化合物黄芩黄酮 A抑制卵巢癌 A2 780细胞生长及对常用抗肿瘤药的体外增效作用。方法 :MTT法测定黄芩黄酮 A的细胞毒作用及与多种抗癌药物作用后对卵巢癌 A2 780细胞的抑制作用 ,集落形成实验观察药物的诱导分化作用 ,PCR- EL ISA试剂盒测定细胞端粒酶活性。结果 :黄芩黄酮 A能抑制 A2 780细胞的增殖 ,其 IC5 0 为 2 0 0 mg/ L,同时黄芩黄酮 A与丝裂霉素 (MMC)、阿霉素 (ADM)、氟尿嘧啶(5 - Fu)、卡铂、羟基喜树碱合用具有相加或协同作用 ,可降低端粒酶活性的表达。结论 :黄芩黄酮 A具有体外抗肿瘤活性 ,对多种化疗药物具有增效作用。
OBJECTIVE: To study the effects of skullcap flavonoids A, a compound extracted from Scutellaria baicalensis Georgi, on the growth of ovarian cancer A2 780 cells and the synergistic effects on antitumor drugs in vitro. Methods: The cytotoxicity of Scutellaria flavonoids A was determined by MTT method and its inhibitory effect on the proliferation of ovarian cancer A278 cells after treated with various anticancer drugs. The colony formation assay was used to observe the induction and differentiation of drugs. The cell end Granzyme activity. Results: Scutellaria flavonoids A could inhibit the proliferation of A2 780 cells with an IC 50 of 200 mg / L. Meanwhile, Scutellaria flavonoids A combined with MMC, ADM and 5 - Fu, , Carboplatin, hydroxycamptothecin combined with additive or synergistic effect, can reduce the expression of telomerase activity. Conclusion: Scutellaria flavonoids A has anti-tumor activity in vitro and has synergistic effect on a variety of chemotherapy drugs.