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目的:建立HPLC法检测虚寒胃痛胶囊中芍药苷和黄芪甲苷的含量测定方法。方法:1芍药苷采用Diamonsil C18(4.6 mm×200 mm,5μm)色谱柱,流动相为甲醇-0.02 mol/L磷酸二氢钾溶液(28∶72);流速1.0mL/min。2黄芪甲苷采用VP-ODS(4.6 mm×150 mm,5μm)色谱柱,流动相为乙腈-水(35∶65)为流动相,流速1.0 mL/min。结果:1芍药苷进样量在0.05109~2.5545μg范围内呈良好线性关系,平均回收率为96.5%(n=6,RSD=0.88%)。2黄芪甲苷进样量在0.4892~12.23μg范围内呈良好线性关系,平均回收率为94.5%(n=6,RSD=3.0%)。结论:该方法准确可靠,无干扰,可用于虚寒胃痛胶囊的质量控制。
Objective: To establish a method for the determination of paeoniflorin and astragaloside Ⅳ in the composition of Deficiency and Stomach of Weifan Weitong Capsules by HPLC. METHODS: Paeoniflorin was separated on a Diamonsil C18 column (4.6 mm × 200 mm, 5 μm) using methanol-0.02 mol / L potassium dihydrogen phosphate solution (28:72) as mobile phase at a flow rate of 1.0 mL / min. 2 Astragaloside was eluted with VP-ODS column (4.6 mm × 150 mm, 5 μm) using acetonitrile-water (35:65) as the mobile phase at a flow rate of 1.0 mL / min. Results: Paeoniflorin injection showed a good linearity within the range of 0.05109 ~ 2.5545μg with an average recovery of 96.5% (n = 6, RSD = 0.88%). 2 Astragaloside injection volume in the range of 0.4892 ~ 12.23μg showed a good linear relationship, the average recovery was 94.5% (n = 6, RSD = 3.0%). Conclusion: The method is accurate and reliable without interference and can be used for the quality control of Deficiency and Stomach Capsule.