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玉米青枯病(茎腐病),其主要致病菌之一——禾谷镰刀菌(Fusariumgraminearum),在PSA人工培养过程中极不易产生孢子。作者采用浅盘培养方法综合产生无性及有性子实体,生产量大,操作简便,效果良好。主要技术要点是:PSA斜面菌种转接在麦粒或高梁粒培养基上,25℃扩繁培养6～7天,至基物长满菌丝,后平铺于白磁盘内(即浅盘培养),给予一定光源的照射处理,并经常喷水,保持一定湿度。经48～72小时即可丰产分生孢子及有性子实体,环境适宜温度18～25℃。
Corn bacterial wilt (stalk rot), one of the major pathogenic bacteria, Fusarium graminearum, is very difficult to produce spores during the artificial cultivation of PSA. The authors used placental culture methods to produce asexual and sexual fruiting bodies in large quantities with large production volume, easy operation and good effect. The main technical points are: PSA bevel transfer in wheat or sorghum particle culture medium, 25 ℃ proliferation propagation 6 to 7 days, until the substrate is covered with mycelium, and then tiled in the white disk (ie shallow tray Training), give a certain light irradiation treatment, and often water, maintain a certain humidity. After 48 to 72 hours to yield conidia and sexual fruiting bodies, the environment suitable temperature 18 ~ 25 ℃.