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为了探讨来源于不同地理位置贾第虫分离株之间的遗传学关系,采用 tim 基因扩增、限制性酶切和 D N A 序列分析方法对来自我国( C1、 C2、、 C H2、 C H3)、柬埔寨( C A M )、澳大利亚( A1、 A2)和美国( B P、 C D C)共9 株蓝氏贾第虫的基因型进行了分析比较。结果表明, A1、 A2 和 C A M 属第1 型( W B); C H2 和 C H3 属第2 型( J H); C1、 C2、 B P和 C D C属第3 型( G S)。本实验结果提示,虫株间遗传学关系并非由地理位置的远近所决定。来源于同一地理位置的虫株其遗传学特性可有很大的差异。相反,来源于地理位置相隔很远的虫株其遗传学特性却可极为相近。贾第虫分离株基因型的确定可为本虫分子系统进化和分子流行病学研究提供重要资料。
In order to explore the genetic relationship between Giardia isolates from different geographical locations, we used tim gene amplification, restriction enzyme digestion and D N A sequence analysis to analyze the genetic relationships among Giardia isolates from China (C1, C2, C H2, C H3 , Genotypes of 9 Giardia lamblia of Cambodia (C A M), Australia (A1, A2) and the United States (B P, C D C) were analyzed and compared. The results showed that A1, A2 and C A M belonged to type 1 (W B); C H2 and C H3 belonged to type 2 (J H); C1, C2, B P and C D C belonged to type 3 ). The results of this experiment suggest that the genetic relationship between insect strains is not determined by the geographical location. Insect strains from the same geographic location can have very different genetic characteristics. In contrast, insect strains derived from geographically distant strains can have very similar genetic properties. The determination of genotypes of Giardia isolates may provide important information for molecular phylogenetic analysis and molecular epidemiology of this species.