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目的 探讨因缺氧引起颈动脉体K+ 流减少而导致颈动脉体神经活性增加的分子学机制。 方法 利用逆转录聚合酶链反应 (RT PCR)评价大鼠颈动脉体的ATP敏感性钾离子 (KATP)通道的mRNA表达。 结果 在大鼠颈动脉体中内向整流性钾离子通道亚家族Kir6 .1的mRNA是存在的 ,而Kir6 .2和磺酰脲受体 (SUR1andSUR2 )则未见。 结论 Kir6 .1mRNA的存在提示KATP通道在颈动脉体感受缺氧应答时可能起重要作用
OBJECTIVE: To investigate the molecular mechanism of increased carotid body nerve activity caused by hypoxia-induced decrease of K + flow in carotid body. Methods Reverse transcription-polymerase chain reaction (RT-PCR) was used to evaluate the mRNA expression of ATP-sensitive potassium channel (KATP) in rat carotid body. RESULTS: Intrinsic potassium channel subfamily Kir6.1 mRNA was present in the carotid body of rats, whereas no Kir6.2 and sulfonylurea receptors (SUR1andSUR2) were found. Conclusion The presence of Kir6.1 mRNA suggests that KATP channel may play an important role in the hypoxic response of carotid body