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目的:探讨麻杏石甘汤配伍对其解热作用的影响。方法:利用混料均匀设计,以麻黄(X1)、苦杏仁(X2)、炙甘草(X3)和石膏(X4)4个组分设计4因素12水平的麻杏石甘汤配比实验。将SD健康雄性大鼠随机分为15组,分别为麻杏石甘汤配比N1~N12组、临床常用方组、模型组、空白组。采用尾静脉注射脂多糖(LPS)造模,造模后每0.5 h记录1次体温,计算体温反应指数(TRI);测定造模后6.0 h血浆前列腺素E2(PGE2)含量;对结果进行二次多项式回归分析。结果:各配伍组与模型组比较,均能不同程度的抑制LPS诱导的大鼠发热,具有极显著性意义(P<0.01);经麻杏石甘汤干预后,血浆PGE2有不同程度的下降;经麻杏石甘汤干预后,TRI有不同程度的下降。回归分析的结果表明,影响TRI的因素为石膏>麻黄>苦杏仁与炙甘草的交互作用。麻黄与体温反应指数为负相关。结论:4因素12水平的混料均匀设计实验结果表明:对于疗热,方中石膏为君药,最佳配比剂量为麻黄0.6 g,苦杏仁7.5 g,甘草7.5 g,石膏26.4 g。
Objective: To investigate the effect of Maxing Shigan Decoction on its antipyretic effect. Methods: Four ingredients and 12 levels of Maxing Shigan Decoction were designed with the four ingredients of ephedra (X1), bitter almond (X2), licorice (X3) and gypsum (X4) The SD healthy male rats were randomly divided into 15 groups: Maxing Shigan Decoction N1 ~ N12 group, clinically used square group, model group and blank group. (LPS) was injected into the caudal vein and body temperature was recorded every 0.5 h after modeling. The body temperature response index (TRI) was calculated. The content of prostaglandin E2 (PGE2) at 6.0 h after modeling was measured. Polynomial Regression Analysis. Results: Compared with the model group, all compatibility groups could inhibit the LPS-induced fever in rats to a certain extent (P <0.01). The plasma PGE2 level decreased with the intervention of Maxingshigan Decoction ; After Maxing Shi Gan Tang intervention, TRI decreased to varying degrees. The results of regression analysis showed that the factors affecting TRI were gypsum> ephedra> bitter almond and Zhigancao interaction. Ephedra and the body temperature response index is negatively correlated. CONCLUSION: The experimental results of uniform design with 4 factors and 12 levels show that the best prescription is Ephedra 0.6 g, bitter almond 7.5 g, licorice 7.5 g and gypsum 26.4 g.