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目的:采用气相色谱(GC)法同时测定人血清中抗癫痫药丙戊酸钠和托吡酯的浓度。方法:以环己酸作内标,采用HP-5毛细管柱(30m×0.32mm,0.25μm);FID检测器,柱温:200℃,进样口温度220℃,检测器温度240℃;不分流进样。载气(高纯氮):4mL.min-1,氢气:35mL.min-1,空气:400mL.min-1。结果:丙戊酸钠和托吡酯的血药浓度分别在10.00～250.00mg.L-1(Y=0.03726C-0.001013,r=0.9997)和10.0～1000.0mg.L-1(Y=0.03166C+0.00201,r=0.9994)范围内线性关系良好;平均回收率(n=15)分别为96.91%和97.71%;丙戊酸钠25.0,100.0,150.0mg.L-1;日内精密度(RSD,n=5)分别为3.8%,4.1%和3.0%,日间RSD(n=5)分别为5.1%,4.9%%和4.4%;托吡酯40.0,200.0和400.0mg.L-1的日内RSD分别为4.2%,2.8%和2.2%(n=5),日间RSD分别为4.1%,3.1%和3.6%(n=5)。结论:本方法操作简单、回收率高、精密度好,适用于临床上常规监测丙戊酸钠和托吡酯的血药浓度。
Objective: To simultaneously determine the concentrations of antiepileptic sodium valproate and topiramate in human serum by gas chromatography (GC). Methods: HP-5 capillary column (30m × 0.32mm, 0.25μm) was used with cyclohexanoic acid as the internal standard. The FID detector was used. The column temperature was 200 ℃, the inlet temperature was 220 ℃ and the detector temperature was 240 ℃. Split injection. Carrier gas (high purity nitrogen): 4mL.min-1, hydrogen: 35mL.min-1, air: 400mL.min-1. Results: The plasma concentrations of sodium valproate and topiramate were in the range of 10.00-250.00 mg.L-1 (Y = 0.03726C-0.001013, r = 0.9997) and 10.0-1000.0 mg.L-1 (Y = 0.03166C + 0.00201 , r = 0.9994). The average recoveries (n = 15) were 96.91% and 97.71% respectively. The contents of sodium valproate 25.0,100.0,150.0 mg.L- Day RSD (n = 5) were 5.1%, 4.9% and 4.4%, respectively. The daily RSDs of topiramate 40.0, 200.0 and 400.0 mg.L-1 were 4.2 %, 2.8% and 2.2%, respectively (n = 5). Daytime RSD was 4.1%, 3.1% and 3.6%, respectively (n = 5). Conclusion: The method is simple, high recovery, good precision, suitable for routine monitoring of plasma sodium valproate and topiramate concentration.