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通过吡咯(Py)与其衍生物——6-吡咯己胺(PyHA)的共聚物聚(吡咯-co-6-吡咯己胺)[poly(Py-co-PyHA)]的合成研究,并采用电化学循环伏安法来考察体系的电化学活性.在缓冲溶液中,由于探针DNA链上的负电荷与共聚物分子链上的正电荷之间存在强烈的静电吸引力,使得DNA能够固定在导电聚合物膜上.实验结果证明,目标DNA和聚吡咯薄膜之间不存在非特异性吸附,而能和探针DNA进行顺利杂交.此结果为以后研究更为敏感的DNA固定及导电聚合物敏感膜提供了实验基础.
The synthesis of poly (pyrrole-co-6-pyrrole) [poly (Py-co-PyHA)], a copolymer of pyrrole and its derivative 6- Chemical cyclic voltammetry to investigate the electrochemical activity of the system.In the buffer solution, due to the strong electrostatic attraction between the negative charge on the DNA strand of the probe and the positive charge on the molecular chain of the copolymer, the DNA can be immobilized Conductive polymer membrane.The experimental results show that there is no nonspecific adsorption between the target DNA and the polypyrrole film, but can be successfully hybridized with the probe DNA.This result is more sensitive to DNA immobilization and conductive polymer sensitivity Membranes provide the experimental basis.