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目的:慢性肾脏病是由于各种原因引起的慢性肾脏疾病(病程在三个月以上)的统称,已成为全球性公共健康问题。本实验旨在研究慢性肾脏病对钛种植体骨结合的影响。方法:采用5/6肾切除法建立CKD小鼠模型,通过细胞培养,并在股骨内植入种植体,于术后2周和4周获取标本,行X-ray片、硬组织切片和生物力学测试。结果:肾病小鼠血清BUN、FGF23、PTH和ALP水平明显升高。在钛种植体表面,肾病小鼠来源骨髓基质细胞(BMSCs)成骨相关基因表达水平(Osx,Col-1,Ocn,和Opn)与假手术组无显著差异;茜素红染色均有矿化结节形成,两组无明显差异。然而种植体植入术后2周,CKD小鼠的种植体-骨接触率(BIC)以及骨结合强度(推出试验)均低于假手术组。讨论:肾病小鼠血清PTH和FGF23的升高,以及VitD的降低可能会影响骨的改建过程,进而引起BIC和骨结合强度的降低。结论:慢性肾脏病影响钛种植体的早期骨结合,但具体影响尚待进一步研究。
Purpose: Chronic kidney disease is a general term for chronic kidney disease (duration of more than three months) due to various causes and has become a global public health issue. The purpose of this experiment was to investigate the effect of chronic kidney disease on the osseointegration of titanium implants. Methods: The model of CKD was established by 5/6 nephrectomy. The cells were implanted into the femur through cell culture. The specimens were obtained at 2 weeks and 4 weeks after operation. X-ray films, hard tissue sections and biological samples Mechanical test. Results: Serum levels of BUN, FGF23, PTH and ALP were significantly increased in nephropathy mice. On the surface of titanium implants, there was no significant difference in osteogenic related gene expression levels (Osx, Col-1, Ocn, and Opn) between bone marrow stromal cells (BMSCs) Nodules formed, no significant difference between the two groups. However, 2 weeks after implantation, the implant-bone contact ratio (BIC) and the bone-cement strength (release test) in CKD mice were lower than those in the sham-operated group. Discussion: Increased serum levels of PTH and FGF23, as well as decreased VitD in nephropathy mice may affect the process of bone remodeling, leading to a decrease in BIC and osseointegration. CONCLUSIONS: Chronic kidney disease affects early bone cement in titanium implants, but the exact effect remains to be further studied.