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目的:了解圆锥角膜基底膜免疫组化的变化,以发现圆锥角膜可能的特异性改变,为探讨其发病机制提供依据。方法:收集圆锥角膜在我院眼科行穿透性角膜移植术的角膜片,用链霉亲和素-生物素法（SP法）检测圆锥角膜、角膜白斑、正常角膜基底膜IV型胶原、Fn、Ln表达的异同,并进行统计学分析。结果:圆锥角膜基底膜IV型胶原,Fn染色较正常角膜显著升高,但与角膜白斑比较无显著性差异。结论:圆锥角膜基底膜的改变可能与创伤愈合有关。提示圆锥角膜原发病变可能在角膜基质细胞。眼科学报2001;17:65～67。
OBJECTIVE: To understand the changes of immunohistochemistry of keratoconus basement membrane in order to find out the possible specific changes of keratoconus and to provide basis for its pathogenesis. Methods: The cornea of corneal keratomileusis penetrating keratoplasty in our hospital was collected. The keratoconus, leukoplakia, type IV collagen of normal corneal basement membrane were detected by streptavidin-biotin method (SP method), Fn , Ln expression similarities and differences, and statistical analysis. Results: Type IV collagen and Fn staining of keratoconus basement membrane was significantly higher than that of normal cornea, but no significant difference compared with corneal leukoplakia. Conclusion: The change of keratoconus basement membrane may be related to wound healing. Tip keratoconus primary lesions may be in corneal stromal cells. Journal of Ophthalmology 2001; 17: 65 ~ 67.