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目的观察内皮祖细胞(endothelial progenitor cells,EPCs)参与肺腺癌新生血管的形成。方法利用携带Lac Z基因的重组腺病毒,以最佳转染浓度转染EPCs,然后将EPCs经肺腺癌动物模型的尾静脉注入,分别在第6、7、8周取肺组织进行病理切片,X-gal染液显色,观察EPCs参与肺癌血管的形成。结果 AD5F35Lac Z转染EPCs的最佳感染复数(multiplicity of infection,MOI)为400;当MOI=400时,AD5F35Lac Z的转染率最大,为97.13±2.08。Lac Z基因转染的EPCs体外培养2周后开始增殖,移植入肺癌动物模型后,第8周参与肺癌组织新生血管的形成。结论 EPCs移植后参与了肿瘤新生血管的形成。
Objective To observe the effect of endothelial progenitor cells (EPCs) on the formation of neovascularization in lung adenocarcinoma. Methods Recombinant adenoviruses harboring Lac Z gene were transfected into EPCs at the optimal transfection concentration. EPCs were injected into the tail vein of animal models of lung adenocarcinoma. Pulmonary tissues were collected at 6, 7 and 8 weeks for histopathology , X-gal stain color, EPCs involved in the formation of lung cancer. Results The optimal multiplicity of infection (MOI) of AD5F35Lac Z transfected EPCs was 400. When MOI = 400, AD5F35Lac Z had the highest transfection rate of 97.13 ± 2.08. EPCs transfected with LacZ gene began to proliferate in vitro two weeks later. After being transplanted into animal model of lung cancer, they were involved in the formation of neovascularization in lung cancer tissue in the eighth week. Conclusion EPCs are involved in the formation of tumor neovascularization after transplantation.