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目的:加密油菜控制硫甙性状QTL区间,并进行QTL整合预测候选基因。方法:利用生物信息学方法根据已知测序的白菜BAC序列信息设计引物,在油菜TN DH群体中进行多态性扩增和定位,并根据加密后构建的遗传连锁图重新检测QTL,进行QTL整合。结果:将根据白菜BAC设计的3对多态性标记成功定位到油菜控制硫甙性状QTL区间,进行QTL整合后将QTL置信区间进一步缩小,并判定了初步的候选基因。结论:充分利用白菜已测序的BAC或者基因组信息,将能加快油菜基础研究的步伐。
OBJECTIVE: Encryption of rapeseed can control the QTL interval of glucosinolate traits and predict the candidate genes by QTL integration. Methods: Using bioinformatics method, primers were designed according to the known sequence of BAC in Chinese cabbage, and amplified and mapped in TN DH population of rapeseed. Based on the genetic linkage map constructed after the encryption, the QTLs were retested for QTL integration . Results: Three pairs of polymorphic markers designed by BAC were successfully mapped to QTLs for trait of glucosinolate trait in rapeseed. The integration of QTLs further narrowed the confidence intervals of QTLs and determined the preliminary candidate genes. Conclusion: Making full use of the sequenced BAC or genomic information in Chinese cabbage will speed up the basic research of rapeseed.