论文部分内容阅读
利用具Pm21的小麦–簇毛麦染色体易位系6AL/6VS为抗病供体,与对白粉病敏感的优良小麦栽培品种京411为轮回亲本杂交、F1个体回交7次,培育了近等基因系(near-isogenic line,NIL)。利用蛋白质组技术,检测了NIL的选择效率,叶子蛋白质组分析发现,NIL中有43.8%的蛋白质组斑点变化倾向于抗病供体亲本6AL/6VS,56.2%的蛋白质组斑点变化倾向于轮回亲本京411。胚根中有41.7%的蛋白质组斑点变化倾向于抗病亲本6AL/6VS,58.3%的蛋白质组斑点变化倾向于轮回亲本京411。通过MS/MS分析,在NIL中有23个蛋白质斑点产生表达变化,它们是:ATP synthase beta subunit、glycosyltransferase family 2 protein、Rab7/RabG-family small GTPase、COP1-interacting protein 7、cryptochrome 1a、ribosomal protein L16、transcription initiation factor TFIID subunit、23.5 kDa heat-shock protein、POR、RIC1、NBS-LRR resistance protein、transcription factor、serine/threonine-protein kinase haspin、F-actin capping protein beta subunit、mitochondrial protein translocase family、maturase K、NADH dehydrogenase subunit F、chloroplast Tha 4-2、mitochondrial translational initiation factor、ATP binding protein、SNF-1 related kinase、heat shock protein 70和lipase family protein。这些蛋白质斑点的功能涉及能量代谢、糖代谢、信号传导、光合反应、DNA和蛋白质合成以及环境胁迫。蛋白质组检测所得结果能反映出NIL的培育与选择效果。
The wheat Allelocaridum translocation line 6AL / 6VS with Pm21 was used as disease-resistant donor, and the elite wheat cultivar Jing 411 with susceptibility to powdery mildew was recurrent parent. F1 individuals were backcrossed 7 times, The near-isogenic line (NIL). The efficiency of selection of NIL was detected by proteomic technology. Leaf proteome analysis revealed that 43.8% of proteomic spots in NIL tended to be resistant to 6AL / 6VS of resistant donor parents, while 56.2% of proteome spots tended to be recurrent parents Beijing 411. 41.7% of proteome spots in radicle tend to change to 6AL / 6VS, and 58.3% of proteome spots tend to reincarnate parents 411. There were 23 protein spots in NIL producing changes of expression by MS / MS analysis, they are: ATP synthase beta subunit, glycosyltransferase family 2 protein, Rab7 / RabG-family small GTPase, COP1-interacting protein 7, L16 transcription initiation factor TFIID subunit 23.5 kDa heat-shock protein POR RIC1 NBS-LRR resistance protein transcription factor serine / threonine-protein kinase haspin F-actin capping protein beta subunit mitochondrial protein translocase family maturase K, NADH dehydrogenase subunit F, chloroplast Tha 4-2, mitochondrial translational initiation factor, ATP binding protein, SNF-1 related kinase, heat shock protein 70 and lipase family protein. The function of these protein spots involves energy metabolism, sugar metabolism, signaling, photosynthesis, DNA and protein synthesis, and environmental stress. The result of proteome detection can reflect the effect of NIL breeding and selection.