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本实验以组按H_2受体拮抗剂甲氰咪胍(Cimetidine)处理低压(0.5大气压)条件下生活的LACA小鼠。观察到:低氧两周后外周RBC数、PCV、Hb骨髓CFU-E数和BFU-E数明显增多,且CFU-E和BFU-E集落较大(集落直径为正常对照的3～5倍,资料未录入);外周血WBC数及骨髓CFU-GM数明显减少。CFU-S向红系分化明显增多,向巨核系、粒系分化明显减少。低氧小鼠同时给予Cimetidine两周后与低氧对照组比较,外周血RBC数、PCV、Hb、CFU-E数、BFU-E数均明显减少。外周血WBC数和Pt数在Cimetidine低剂量组(1mg/天)升高,但随着剂量的增加又有降低趋势(无统计学意义)。Cimetidine使CFU-GM数较低氧对照组明显增多,但随着剂量的增加CFU-GM增多的幅度逐渐减少。高浓度的Cimetidine(2-4mg/天)使CFU-S数较低氧对照组明显减少。CFU-S在Gmetidine的作用下向红系分化明显减少。而向粒系和巨核系的分化相对增多。
In this study, LACA mice were treated with Cimetidine, a H 2 receptor antagonist, at low pressure (0.5 atm). It was observed that the numbers of CFU-E and BFU-E in peripheral RBC, PCV and Hb bone marrow increased significantly after hypoxia for two weeks, and the CFU-E and BFU-E colonies were larger (the diameter of colony was 3-5 times of the normal control , Data not included); WBC number of peripheral blood and bone marrow CFU-GM number decreased significantly. CFU-S erythroid differentiation was significantly increased, to the megakaryocyte, granulocyte differentiation was significantly reduced. Hypoxic mice given Cimetidine two weeks later with the hypoxic control group, peripheral blood RBC count, PCV, Hb, CFU-E number, BFU-E number were significantly reduced. The number of WBC and Pt in peripheral blood increased in Cimetidine low-dose group (1mg / day), but decreased with increasing dose (not statistically significant). Cimetidine significantly increased the number of CFU-GMs in the lower oxygen control group, but decreased gradually with increasing doses of CFU-GM. High concentrations of Cimetidine (2-4 mg / day) significantly reduced the number of CFU-S controls compared to the lower oxygen controls. CFU-S significantly reduced erythroid differentiation under the action of Gmetidine. While the relative differentiation of granulosa and megakaryocyte increased.