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目的:观察丹参酮ⅡA(TanⅡA)联合5-氟尿嘧啶(5-FU)对人胃癌SGC7901细胞的增殖抑制和诱导凋亡作用,及其与突变型p53蛋白表达变化的关系。方法:不同浓度的TanⅡA单独及联合应用10.0 mg/L的5-FU作用于SGC7901细胞24、48和72 h,MTT法检测细胞活力,HO-ECHST染色法检测细胞凋亡;不同浓度的TanⅡA单独及联合10.0 mg/L的5-FU作用于SGC-7901细胞48 h后,免疫细胞化学法检测突变型p53蛋白的表达。结果:实验所选各种浓度TanⅡA均能抑制SGC7901细胞的增殖并诱导其凋亡,且呈时间和剂量依赖性(P值均<0.01),10.0 mg/L TanⅡA作用细胞72 h后,其抑制率及凋亡率分别达47.17%和45.63%。与单用5-FU相比,联合应用TanⅡA细胞增殖抑制率和凋亡率显著增加(P均<0.01),10.0 mg/L TanⅡA联合5-FU作用细胞72 h后,其抑制率及凋亡率分别为65.51%和57.28%。不同浓度的TanⅡA单独及联合应用5-FU作用48 h后,突变型p53表达呈剂量依赖性降低(P均<0.01)。结论:TanⅡA可显著增强5-FU对SGC7901细胞的增殖抑制和诱导凋亡作用,该效应可能与其抑制突变型p53蛋白的表达有关。
Objective: To observe the effects of Tan Ⅱ A combined with 5-fluorouracil (5-FU) on the proliferation and apoptosis of human gastric cancer cell line SGC7901 and its relationship with the expression of mutant p53 protein. METHODS: SGC7901 cells were treated with different concentrations of TanⅡA alone and in combination with 10.0 mg / L 5-FU for 24,48 and 72 h respectively. Cell viability was measured by MTT assay and apoptosis was detected by HO-ECHST staining. Different concentrations of TanⅡA SGC-7901 cells were treated with 10.0 mg / L 5-FU for 48 h, and the expression of mutant p53 protein was detected by immunocytochemistry. Results: TanIIA at various concentrations was able to inhibit proliferation and induce apoptosis of SGC7901 cells in a time and dose-dependent manner (all P <0.01). After treated with 10.0 mg / L TanⅡA for 72 h, The rates of apoptosis and apoptosis were 47.17% and 45.63% respectively. Compared with 5-FU alone, the proliferation and apoptosis rates of TanⅡA cells were significantly increased (P <0.01), and the inhibitory rates and apoptotic rates of Tan Ⅱ A combined with 5-FU treated with 10.0 mg / L 72 h The rates were 65.51% and 57.28% respectively. The expression of mutant p53 was decreased in a dose-dependent manner (P <0.01) after treated with 5-FU alone or in combination with different concentrations of TanⅡA for 48 h. Conclusion: TanⅡA can significantly enhance the inhibitory effect of 5-FU on proliferation and induce apoptosis of SGC7901 cells, which may be related to the inhibition of mutant p53 protein expression.