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目的：观察ＰＤＧＦ对鼠成骨细胞增殖能力的影响及其与ＢＭＰ、ｂＦＧＦ联合应用对细胞的生长有否协同作用。方法：酶消化分段收集法分离培养出鼠成骨细胞，利用ＭＴＴ法检测活细胞产生甲月替结晶的含量多少，反映细胞的增殖能力和生长情况。结果：ＰＤＧＦ能促进成骨细胞的增殖生长，其有效浓度为１～１００ｎｇ／ｍｌ，以１００ｎｇ／ｍｌ作用最大；ＰＤＧＦ与ＢＭＰ、ｂＦＧＦ联合应用可明显增强成骨细胞的增殖能力。结论：ＰＤＧＦ可以刺激成骨细胞的增殖，且与ＢＭＰ、ｂＦＧＦ有协同作用。ＭＴＴ法是测定成骨细胞增殖能力较理想的方法
OBJECTIVE: To observe the effect of PDGF on the proliferation of rat osteoblasts and its synergistic effect with BMP and bFGF on the cell growth. METHODS: Rat osteoblasts were isolated and cultured by enzymatic digestion and fractionated method. MTT assay was used to determine the amount of formazan crystals produced by living cells, which reflected the proliferation and growth of cells. Results: PDGF could promote the proliferation and proliferation of osteoblasts. The effective concentration of PDGF was 1 ~ 100ng / ml, and the effect was greatest at 100ng / ml. Combination of PDGF with BMP and bFGF could significantly enhance the proliferation of osteoblasts. Conclusion: PDGF can stimulate the proliferation of osteoblasts and has synergistic effect with BMP and bFGF. MTT assay is an ideal method for the determination of osteoblast proliferation