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甲型肝炎患者26例血清以二巯基乙醇处理,用微量补体结合试验比较处理前后的补体结合反应滴度,以检测甲型肝炎IgM抗体。结果经处理后的滴度下降4倍或4倍以上者14例,阳性率53%。9例双(三)份血清中有2例甲型肝炎IgM抗体可持续存在一年左右。从而说明微量补体结合试验可以作为诊断甲型肝炎近期感染的参考。另外,采用粪抗原包被的微孔板,对上述14例血清作酶联免疫吸附测定,结果不论急性期或恢复期血清甲型肝炎IgM和IgG抗体均为阳性,未能达到早期血清学诊断的目的。其原因有
Twenty-six patients with hepatitis A were treated with dimercaptoethanol, and the complement fixation reaction titers before and after treatment were compared with the trace complement fixation test to detect hepatitis A IgM antibodies. Results Treated titers dropped 4 times or more in 14 cases, the positive rate of 53%. Two cases of hepatitis B IgM antibodies in two (three) sera of nine cases persisted for about one year. This shows that trace complement fixation test can be used as a reference diagnosis of recent hepatitis A infection. In addition, the use of fecal antigen coated microplates on the above-mentioned 14 cases of serum enzyme-linked immunosorbent assay, regardless of acute or convalescent serum hepatitis A IgM and IgG antibodies were positive, failed to achieve early serological diagnosis the goal of. The reason is