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目的 :探讨铅对海马 LTP的影响与海马不同亚区神经元型一氧化氮合酶 ( n NOS)变化的关系。方法 :1 5只大鼠饮用含 0 .2 %酯酸铅 3 m后 ,作为染铅组进行实验。采用原子吸收法测定血液与海马中铅含量 ;用免疫组化法研究大鼠海马不同亚区 n NOS的表达情况。结果 :染铅组大鼠血液与海马中铅含量明显高于对照组 (血铅为 1 50 .69± 52 .1 4 ng/ml比 5.1 6± 1 .1 5ng/ml;海马铅为 1 1 69.2 7± 1 68.0 6ng/g比 1 99.63± 4 3 .1 4 ng/g,P<0 .0 5) ;染铅组大鼠海马 CA1区 n NOS阳性细胞数目 ( 49.56± 6.70 )明显少于对照组 ( 64 .56± 1 2 .4 6,P<0 .0 5) ,在 CA3区无明显改变。结论 :铅对海马 LTP的影响可能与染铅后海马各区 n NOS的不同变化有关
AIM: To investigate the relationship between the effects of lead on hippocampal LTP and the changes of neuronal nitric oxide synthase (nNOS) in different subregions of hippocampus. Methods: Fifteen rats were drank with 0.2% lead for 3 minutes and then tested as lead-exposed group. The contents of lead in blood and hippocampus were determined by atomic absorption spectrometry. The expression of nOS in different sub-regions of hippocampus was detected by immunohistochemistry. Results: The levels of lead in the blood and hippocampus of the lead-exposed rats were significantly higher than those of the control rats (blood lead was 1 50.69 ± 52.14 ng / ml vs 5.1 6 ± 1. 69.2 7 ± 1 68.0 6ng / g vs 99.63 ± 4.31.4 ng / g, P <0.05). The number of nNOS positive cells in hippocampal CA1 subregion of the lead-exposed rats was significantly lower than that of the control rats (49.56 ± 6.70) The control group (64.56 ± 12.46, P <0.05) showed no significant changes in CA3 area. CONCLUSION: The effect of lead on hippocampal LTP may be related to the different changes of nOS in different regions of hippocampus after lead exposure