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Perturbation of the circadian rhythm damages the biological characteristics of cells and leads to their dysfunction.Rev-erbα,an important gene in the transcription-translation loop of circadian rhythm,is involved in regulating the balance between pro-inflammation and anti-inflammation.The disruption of this balance in human endometrial stroma cells (hESCs) destroys their biological behavior function in maintaining the menstrual cycle and embryonic implantation.Whether pharmacological modulation of Rev-erbα affects the inflammation of hESCs remains unclear.In this study,we treated hESCs with lipopolysaccharide (LPS) and found that LPS treatment increased the mRNA levels of pro-inflammatory cytokines,such as interleukin (IL)-1β,IL-6,IL-8,IL-18,and TN Fα,and the secretion of IL-6.SR9009,a Rev-erbα agonist,significantly alleviated the LPS-induced production of pro-inflammatory cytokines in hESCs.Meanwhile,knockdown of Rev-erbα increased the expressions of IL-1β,IL-6,and IL-8,accompanied by an increased mRNA level of the core clock gene Bmal1.Weste blot analysis showed that SR9009 inhibited the expression of tolllike receptor 4 (TLR4) and the activation of NF-κB induced by LPS.All these findings suggested that pharmacological activation of Rev-erbα attenuated the LPS-induced inflammatory response of hESCs by suppressing TLR4-regulated NF-κB activation.This study may provide a strategy for preventing inflammation-related endometrial dysfunction and infertility or recurrent implantation failure.