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目的分析经皮染毒反复束缚对大鼠生长的影响。方法 8周龄SD大鼠随机分为束缚组和对照组,每组20只,雌雄各半。束缚组大鼠模拟经皮染毒试验每天束缚固定6 h,连续91 d,对照组不作上述处理。观察两组大鼠临床症状、体重和摄食量变化,试验结束时进行血液学、血清生化和尿液生化检测,并作大体解剖和病理组织学检查,采用广义估计方程比较两组间差异。结果广义估计方程统计结果显示,无论雌雄大鼠,体重变化组别和试验时间存在交互作用(P<0.05),且束缚组雄性大鼠体重低于对照组(P<0.05)。进一步分析发现,雄性大鼠体重从试验第4周开始低于对照组(P<0.05),并且组别与试验时间间出现交互作用(P<0.05);雌性大鼠从试验第8周开始,组别与试验时间间出现交互作用(P<0.05)。束缚组雌性大鼠红细胞(RBC)、血红蛋白(HGB)、血容积比(HCT)和雄性大鼠RBC和HGB水平均高于对照组(P<0.05),但无生物学意义;两组间其他观察指标差异均无统计学意义(P>0.05)。结论经皮染毒反复束缚影响大鼠体重的增长,且雄性大鼠的敏感性高于雌性大鼠。
Objective To analyze the effect of repeated tethering of transdermal drug on the growth of rats. Methods 8-week-old SD rats were randomly divided into shackles group and control group, each group 20, male and female. The rats in shackles group were shackled and fixed for 6 hours every day for 91 days. The control group was not treated as above. The changes of clinical symptoms, body weight and food intake of the two groups were observed. At the end of the experiment, hematology, serum biochemistry and urine biochemical tests were performed. Gross anatomy and histopathological examination were performed. The generalized estimation equation was used to compare the differences between the two groups. Results The results of generalized estimating equation showed that there was interaction between body weight change group and test time in male and female rats (P <0.05), and the weight of male rats in shackles group was lower than that in control group (P <0.05). Further analysis showed that the body weight of male rats was lower than that of the control group from the 4th week of the experiment (P <0.05), and there was interaction between the groups and the experimental time (P <0.05). From the 8th week of the experiment, Interaction between groups and experimental time (P <0.05). The levels of RBC, HGB, HCT and RBC and HGB in sham group were higher than those in control group (P <0.05), but no biological significance There was no significant difference between the observation indexes (P> 0.05). Conclusion Repeated tetanic dermal administration affects the growth of body weight in rats, and the sensitivity of male rats is higher than that of female rats.