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白细胞介素2(IL-2)是重要的免疫调节因子。对IL-2的检测,国外一般采用依赖IL-2的T细胞株CTLL细胞进行测定。该细胞在国内来源困难,不易维持。国内亦有用鼠胸腺细胞增殖试验来测定IL-2者,但由于不能消除IL-1的非特异影响,因此,该方法受一定局限。本文参照Christine的报道,用经Con-A(4μg/ml)诱导及氢化可的松(0.04μg/ml)处理的鼠胸腺细胞(HCIT)进行IL-2测定,并同时用CTLL-2细胞测定方法进行比较,结果证实:用HCIT细胞测定IL-2与用CTLL-2细胞测定两种方法有很好的相关性、相关系数r=0.94。但用CTLL细胞比用HCIT细胞较敏感。在本试验条件下,前者敏感量为0.4IL-2活性单位,后者为1.3IL-2活性单位。因此用HCIT细胞进行IL-2测定,也可以达到特异(对IL-1不发生反应),灵敏、重复性好的效果。且由于胸腺细胞容易获取,操作技术简便、易于掌握,是一种在没有CTLL细胞情况下测定IL-2的较好的方法。
Interleukin 2 (IL-2) is an important immunomodulatory factor. For the detection of IL-2, IL-2-dependent T cell line CTLL cells are generally used abroad for determination. The cell source in the country is difficult to maintain. There are also domestic rat thymocyte proliferation test to determine IL-2, but because it can not eliminate the non-specific effects of IL-1, therefore, this method is subject to certain limitations. IL-2 assay was performed with murine thymocytes (HCIT) treated with Con-A (4 μg / ml) and hydrocortisone (0.04 μg / ml) as assayed by Christine and simultaneously assayed with CTLL-2 cells The results showed that there was a good correlation between IL-2 measured by HCIT cells and CTLL-2 cells, the correlation coefficient was 0.94. However, CTLL cells are more sensitive than HCIT cells. Under the experimental conditions, the former sensitive amount of 0.4IL-2 activity unit, which is 1.3IL-2 activity unit. Therefore, HCIT cells for IL-2 measurement, but also can achieve specific (no response to IL-1), sensitive, reproducible results. And because thymus cells are easy to access, the technique is simple and easy to master, and it is a better method to determine IL-2 in the absence of CTLL cells.